The Microfollicle: A Model of the Human Hair Follicle for In Vitro Studies

November 2020 in “ In Vitro Cellular & Developmental Biology - Animal ”

Beren Ataç, Flora Marta Kiss, Tobias Lam, Beatrix Fauler, Clemens Edler, Ping Hu, Thi Phuong Tao, Marian Jädicke, Isabel Rütschle, Reza P. Azar, Scott R. Youngquist, Thorsten Mielke, Uwe Marx, Roland Lauster, G Lindner, Teresa DiColandrea

microfollicle organoid model hair follicle 3D differentiation platform keratin expression gene expression minoxidil hair-growth compound Rogaine

Studysummary This study explored the use of "microfollicles," a human hair follicle organoid model, as an in vitro tool for testing hair-growth substances, finding up to 75% overlap in gene expression changes with human clinical biopsy responses to minoxidil, indicating its potential for pre-clinical substance testing.

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This review discusses the historical development and current challenges in in vitro hair follicle culture, highlighting a need for advanced models using cell lines and tissue engineering, and reports no new results.

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Androgens Trigger Different Growth Responses in Genetically Identical Human Hair Follicles in Organ Culture Reflecting Their Epigenetic Diversity in Life

research Androgens Trigger Different Growth Responses in Genetically Identical Human Hair Follicles in Organ Culture Reflecting Their Epigenetic Diversity in Life

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research In Vitro Skin Models to Study Epithelial Regeneration from the Hair Follicle

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research Endogenous Human Skin Equivalent Promotes In Vitro Morphogenesis of Follicle-Like Structures

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This study demonstrated that an engineered human skin equivalent can generate follicle-like structures resembling fetal skin, highlighting the role of the dermal compartment in directing epithelial cell fate in vitro.

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130 citations , August 2015 in “Experimental Dermatology”

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This review examines the literature on the molecular and cellular makeup of the hair follicle niche and how it influences stem cell behavior during hair regeneration, but does not provide new experimental results.

research Strategies to Enhance Epithelial-Mesenchymal Interactions for Human Hair Follicle Bioengineering

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This article reviews methods to enhance epithelial–mesenchymal interactions for hair follicle bioengineering but does not present new experimental results; it emphasizes optimizing combinations for successful regeneration.

research Minoxidil Activates β-Catenin Pathway in Human Dermal Papilla Cells: A Possible Explanation for Its Anagen Prolongation Effect

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This study found that topical minoxidil modestly extended the anagen phase in mice by activating β-catenin activity in dermal papilla cells.

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research The Cycling Hair Follicle as an Ideal Systems Biology Research Model

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research A Cell-Based System for Screening Hair Growth-Promoting Agents

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This review summarizes fundamental concepts and recent advancements in hair follicle biology, including insights from mouse models into broader molecular and cellular processes relevant to regeneration and development.

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research Hair Follicle-Specific Keratins And Their Diseases

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More research is needed to understand how hair keratins work and their role in hair disorders.

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397 citations , February 2004 in “British Journal of Dermatology”

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research Trichohyalin Mechanically Strengthens the Hair Follicle

108 citations , October 2003 in “Journal of biological chemistry/The Journal of biological chemistry”

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78 citations , August 2002 in “Experimental Dermatology”

This study describes a simplified method for isolating and cultivating human hair follicle papillae that can be easily executed by new lab personnel.

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231 citations , December 1999 in “Journal of Investigative Dermatology”

This study found that the volume of a hair follicle's dermal papilla is strongly correlated with its size, primarily due to the number of cells it contains and the volume of extracellular matrix.

research Minoxidil Upregulates the Expression of Vascular Endothelial Growth Factor in Human Hair Dermal Papilla Cells

192 citations , March 1998 in “British Journal of Dermatology”

This study found that minoxidil significantly increases VEGF mRNA and protein expression in dermal papilla cells, suggesting a role in enhancing hair follicle vascularization in androgenetic alopecia.

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This study found that distinct keratin profiles enabled the evaluation of epithelial differentiation in human hair follicles, with mesenchymal cells playing a critical role in epidermoid differentiation of follicular cells.