Development, Validation, And Application Of An RP-HPLC Method For Concurrent Quantification Of Minoxidil And Finasteride In A Topical Solution For Hair Regrowth

October 2020 in “ Annales pharmaceutiques françaises ”

Dinesh O. Shah, D. K. Jha, P.D Amin

Minoxidil Finasteride RP-HPLC Reverse Phase High Performance Liquid Chromatography Active Pharmaceutical Ingredients pKa log P Rogaine Propecia

TLDR The document concludes that a new method for measuring Minoxidil and Finasteride in hair regrowth solution is better because it considers separation theory and the properties of the ingredients. The mix of Methanol and water and the pH level greatly impact the measurement time and clarity.

The document presents a study on the concurrent quantification of Minoxidil (MXL) and Finasteride (FNS) using a Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method. The method was optimized considering factors such as wavelength, the composition of the mobile phase, and pH of buffer. The study concluded that this method is superior to others as it optimizes based on the theoretical aspects of separation and the physicochemical properties of the Active Pharmaceutical Ingredients (APIs) such as pKa and log P. The ratio of Methanol: Milli-Q water and the pH of the mobile phase significantly affected the retention time and resolution of the two APIs.

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