Development, Validation, And Application Of An RP-HPLC Method For Concurrent Quantification Of Minoxidil And Finasteride In A Topical Solution For Hair Regrowth

March 2021 in “ Annales pharmaceutiques françaises ”

Dinesh O. Shah, D. K. Jha, P.D Amin

Minoxidil Finasteride RP-HPLC Reverse Phase High Performance Liquid Chromatography Active Pharmaceutical Ingredients pKa log P Rogaine Propecia

TLDR The document concludes that a new method for measuring Minoxidil and Finasteride in hair regrowth solution is better because it considers separation theory and the properties of the ingredients. The mix of Methanol and water and the pH level greatly impact the measurement time and clarity.

The document presents a study on the concurrent quantification of Minoxidil (MXL) and Finasteride (FNS) using a Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method. The method was optimized considering factors such as wavelength, the composition of the mobile phase, and pH of buffer. The study concluded that this method is superior to others as it optimizes based on the theoretical aspects of separation and the physicochemical properties of the Active Pharmaceutical Ingredients (APIs) such as pKa and log P. The ratio of Methanol: Milli-Q water and the pH of the mobile phase significantly affected the retention time and resolution of the two APIs.

View this study on sciencedirect.com →

Discuss this study in the Community →

Cited in this study

26 / 26 results

research Solid Effervescent Formulations as a New Approach for Topical Minoxidil Delivery

33 citations , January 2017 in “European Journal of Pharmaceutical Sciences”

Effervescent formulations may improve minoxidil delivery, increasing effectiveness and reducing applications needed.

research Minoxidil Topical Treatment May Be More Efficient If Applied on Damp Scalp Compared to Dry Scalp

13 citations , June 2016 in “Dermatologic Therapy”

Minoxidil works better when applied on a damp scalp than a dry one.

research Preparation of Finasteride Capsules-Loaded Drug Nanoparticles: Formulation, Optimization, In Vitro, and Pharmacokinetic Evaluation

28 citations , February 2016 in “International Journal of Nanomedicine”

Finasteride capsules with nanoparticles improve drug delivery, solubility, stability, and effectiveness.

research Computational Design and Development of a Novel Voltammetric Sensor for Minoxidil Detection Based on Electropolymerized Molecularly Imprinted Polymer

39 citations , March 2015 in “Journal of Electroanalytical Chemistry”

New sensor detects minoxidil accurately and effectively.

research Topical Minoxidil Fortified with Finasteride: Maintenance of Hair Density After Replacing Oral Finasteride

32 citations , January 2015 in “Indian Dermatology Online Journal”

Minoxidil and finasteride combo maintains hair density effectively.

research Stability-Indicating Micellar Electrokinetic Chromatography Method for Determination of Finasteride in Pharmaceutical Preparations

5 citations , June 2012 in “Journal of Liquid Chromatography & Related Technologies”

Method accurately measures finasteride concentration in medicine.

research Alopecia: Evaluation and Treatment

76 citations , July 2011 in “Clinical, Cosmetic and Investigational Dermatology”

The document concludes that proper diagnosis and FDA-approved treatments for different types of hair loss exist, but treatments for severe cases often fail and future improvements may focus on hair follicle stem cells.

research Effect of Charge Carrier Lipid on Skin Penetration, Retention, and Hair Growth of Topically Applied Finasteride-Containing Liposomes

11 citations , April 2011 in “Biomolecules & Therapeutics”

research Solubility of Minoxidil in Methanol, Ethanol, 1-Propanol, 2-Propanol, 1-Butanol, and Water from 278.15 to 333.15 K

24 citations , April 2011 in “Journal of Chemical & Engineering Data”

Minoxidil dissolves best in methanol and least in water.

research Transcutol-Containing Vesicles for Topical Delivery of Minoxidil

51 citations , March 2011 in “Journal of Drug Targeting”

Transcutol-containing vesicles improve minoxidil's skin penetration and hair growth promotion.

research Hair Growth and Rejuvenation: An Overview

81 citations , June 2010 in “Journal of Dermatological Treatment”

The document concludes that minoxidil and finasteride are proven for hair growth, herbal remedies show promise, but more research is needed to confirm their effectiveness.

research Male Androgenetic Alopecia

100 citations , May 2010 in “Expert Opinion on Pharmacotherapy”

Hair loss in men treated best with early medication or transplant, new treatments researched.

research Penetration Enhancer-Containing Vesicles as Carriers for Cutaneous Delivery of Minoxidil

128 citations , October 2009 in “International Journal of Pharmaceutics”

PEVs improve minoxidil skin penetration, increasing hair growth.

research Voltammetric Reduction of Finasteride at Mercury Electrode and Its Determination in Tablets

21 citations , May 2008 in “Talanta”

New, cheaper method measures finasteride in tablets accurately and quickly.

research Androgenetic Alopecia: Clinical Features, Classification, and Treatments

103 citations , June 2007 in “Endocrinology and Metabolism Clinics of North America”

Male pattern hair loss is genetic and influenced by hormones, with treatments like minoxidil and surgery available.

research Development and Validation of a New Gas Flame Ionization Detector Method for the Determination of Finasteride in Tablets

13 citations , May 2006 in “Analytical Biochemistry”

New method accurately measures finasteride in tablets.

research Determination of Finasteride in Tablet Form by Liquid Chromatography and Its Analytical Method Validation

11 citations , January 2006 in “Analytica Chimica Acta”

Validated method reliably measures finasteride in tablets using liquid chromatography.

research Development and Validation of a Procedure for the Determination of Minoxidil in Hair-Regrowth Formulations Using Two Variants of Capillary Zone Electrophoresis

14 citations , June 2005 in “Farmaco”

A method was created in 2005 to identify minoxidil, a hair growth ingredient, in products using two types of capillary zone electrophoresis, and it found that most products had about 2% minoxidil.

research Rapid Determination of Minoxidil in Human Plasma Using Ion-Pair HPLC

20 citations , October 2004 in “Journal of Pharmaceutical and Biomedical Analysis”

Quick method to measure minoxidil in blood, accurate and useful for labs.

research Minoxidil: Mechanisms of Action on Hair Growth

397 citations , February 2004 in “British Journal of Dermatology”

Minoxidil boosts hair growth by opening potassium channels and increasing cell activity.

research Bioavailability Assessment of Topical Delivery Systems: Effect of Vehicle Evaporation Upon In Vitro Delivery of Minoxidil from Solution Formulations

44 citations , October 1989 in “International Journal of Pharmaceutics”

Minoxidil's effectiveness decreases as vehicle evaporates; concentration and thermodynamic activity matter.

research Electrochemical Studies on Minoxidil and Its Determination in Tablets by Differential-Pulse Polarography

25 citations , January 1983 in “Analyst”

Method measures minoxidil in tablets accurately and easily.