Ovine Hair Follicle Stem Cells Derived From Single Vibrissae Reconstitute Haired Skin

The study demonstrated that ovine hair follicle stem cells (HFSCs) derived from single vibrissae could reconstitute haired skin, highlighting their potential in hair regeneration therapies. Researchers isolated and cultured these HFSCs using microdissection and organ culture techniques, preserving their natural niche and stem cell activities. The keratinocytes expressed key HFSC markers and exhibited robust proliferation and differentiation capabilities. When grafted with neonatal dermal cells into nude mice, they successfully formed new hair follicles and sebaceous glands. This method provided a stable way to obtain ovine HFSCs, potentially enhancing wool production and serving as a cell bank for physiological and genetic studies in larger mammals. The study suggested these cells could be a valuable resource for regenerative medicine and bioengineering.