Epidermal Structure Created by Canine Hair Follicle Keratinocytes Enriched with Bulge Cells in a Three-Dimensional Skin Equivalent Model In Vitro: Implications for Regenerative Therapy of Canine Epidermis
January 2013
in “
Veterinary dermatology
”
TLDR Keratinocytes from dog hair follicles can create a functional skin layer in a lab model, useful for dog skin therapy.
The study demonstrated that keratinocytes enriched with bulge cells from canine hair follicles could successfully create an interfollicular epidermis in a three-dimensional skin equivalent model in vitro within 2 weeks. Using four healthy beagle dogs, the researchers isolated and cultured these keratinocytes, which showed high proliferative capacity and characteristics of epithelial stem cells. The resulting epidermal structure comprised four to five living cell layers with a horny layer on top, and various keratins and desmogleins were appropriately localized within the layers. This model showed potential for regenerative therapy of canine skin.
