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Did you mean Single-cell RNA sequencing?

Glossary Single-cell RNA sequencing

examines gene expression in individual cells for detailed insights

Single-cell RNA sequencing (scRNA-seq) is a powerful technique that allows scientists to examine the gene expression profiles of individual cells. This method provides detailed insights into the cellular diversity within a tissue, revealing how different cells function and interact, which is crucial for understanding complex biological processes and diseases like cancer or alopecia.

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research Single-Cell Sequencing Technology in Ruminant Livestock: Challenges and Opportunities

5 citations , May 2024 in “Current Issues in Molecular Biology”

This review highlights advancements in applying single-cell sequencing to cattle, sheep, and goats, noting its potential to elucidate cellular diversity and improve traits affecting livestock health and productivity, despite challenges in cell population annotation and spatial resolution in these species.

Differences in Cell Subsets Between Sun-Exposed and Unexposed Skin: Preliminary Single-Cell Sequencing and Biological Analysis from a Single Case

research Differences in cell subsets between sun-exposed and unexposed skin: preliminary single-cell sequencing and biological analysis from a single case

3 citations , October 2024 in “Frontiers in Medicine”

This study investigated single-cell changes in photoaged skin, revealing distinct cell clusters and increased activity in PD-L1 and PD-1 pathways in sun-exposed areas, enhancing understanding of UVA-induced skin damage and potential prevention targets for photoaging and UV-induced skin cancers.

Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Potentially Related to the Fineness of Cashmere Fiber

research Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber

3 citations , November 2021 in “Frontiers in Genetics”

This study suggests that the CXCL8 gene may regulate cashmere fineness in Liaoning cashmere goats, providing new insights into the cellular mechanisms of cashmere growth and quality.

research Single-cell sequencing reveals the new existence form of dermal papilla cells in the hair follicle regeneration of cashmere goats

2 citations , February 2022 in “Genomics”

This study used cashmere goats to map the differentiation of dermal papilla stem cells and identified key intermediate cell states involved in hair follicle regeneration.

10× Genomics Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Potentially Related to the Fineness of Cashmere Fiber

research 10×Genomics Single-cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber

1 citations , November 2020 in “Research Square (Research Square)”

This study identified genes that may regulate cashmere fineness in Liaoning Cashmere Goats by analyzing skin cell types and exploring gene expression in secondary hair follicle dermal papilla cells.

research Single-cell sequencing combined with spatial transcriptomics reveals the characteristics of follicle-targeted inflammation patterns in primary cicatricial alopecia

July 2025 in “Cell & Bioscience”

Specific immune cells and pathways contribute to hair follicle inflammation and hair loss, suggesting potential treatments for lichen planopilaris.

Single-Cell Sequencing Reveals the Intermediate Cell State and Function of Dermal Papilla Cells in the Hair Follicle Cycle of Cashmere Goats

research Single-cell sequencing reveals the intermediate cell state and function of dermal papilla cells in the hair follicle cycle of cashmere goats

May 2020 in “Research Square (Research Square)”

This study used cashmere goats to reveal distinct intermediate states of dermal papilla cells, each with specific roles in hair growth, shedding, and regeneration.

research Single-Cell RNA Sequencing Reveals Cellular and Transcriptional Changes Associated With M1 Macrophage Polarization in Hidradenitis Suppurativa

22 citations , August 2021 in “Frontiers in medicine”

This study found that monocytes/macrophages with a pro-inflammatory M1-like phenotype may play a crucial role in the pathogenesis of hidradenitis suppurativa, suggesting potential therapeutic targets.

research Single‐Cell RNA Sequencing Redefines Macrophage Heterogeneity and Function in Wound Healing

November 2025 in “Wound Repair and Regeneration”

This review highlights single-cell sequencing's role in understanding macrophages' diverse functions and subtypes in skin wound healing, emphasizing macrophages' importance in tissue restoration and identifying key genes involved in the process.

Single-Cell RNA Sequencing Highlights a Contribution of Human Amniotic Mesenchymal Stem Cells-Derived Exosomes to Androgenetic Alopecia

research Single‐Cell RNA Sequencing Highlights a Contribution of Human Amniotic Mesenchymal Stem Cells‐Derived Exosomes to Androgenetic Alopecia

July 2025 in “The FASEB Journal”

This study reported that exosomes derived from human amniotic mesenchymal stem cells (hAMSC-exo) accelerated hair growth in androgenetic alopecia mice by enhancing signals between hair follicle cells and improving cellular environments, particularly protecting against dihydrotestosterone-induced damage via Wnt/β-catenin signaling.

research Molecular heterogeneity of quiescent melanocyte stem cells revealed by single‐cell RNA‐sequencing

April 2024 in “Pigment cell & melanoma research”

This study explored the diversity of melanocyte stem cell subpopulations in the hair follicles of adult female mice and identified novel groups with distinct immune privilege regulation, suggesting a heterogeneous landscape that future research should consider.

Fibroblast Heterogeneity and Functions: Insights from Single-Cell Sequencing in Wound Healing, Breast Cancer, Ovarian Cancer, and Melanoma

research Fibroblast heterogeneity and functions: insights from single-cell sequencing in wound healing, breast cancer, ovarian cancer and melanoma

March 2024 in “Frontiers in genetics”

This review discusses the insights gained from single-cell RNA sequencing of fibroblasts in various cancers and wound healing, highlighting differences in gene expression and novel interactions.

research Global Single-Cell Sequencing Landscape of Adipose Tissue of Different Anatomical Site Origin in Humans

May 2023 in “Stem Cells International”

In this study, researchers used single-cell RNA sequencing to identify cell types and molecular differences in subcutaneous adipose tissue from various anatomical sites, suggesting that certain subpopulations of human adipose stem cells might improve the treatment of chronic refractory wounds.

research Functional interrogation of lymphocyte subsets in alopecia areata using single-cell RNA sequencing

23 citations , July 2023 in “Proceedings of the National Academy of Sciences”

In this study using a mouse model and human data, researchers found that CD8+ T cells are central to driving alopecia areata, while regulatory T cells offer some protection, highlighting CD8+ T cells as key targets for future therapy development.

research Construction of transcriptome atlas of white yak hair follicle during anagen and catagen using single-cell RNA sequencing

8 citations , December 2022 in “BMC Genomics”

This study revealed gene expression patterns in yak hair follicles during different growth phases, enhancing the understanding of cell fate specialization and providing insights for yak villus development.

Humanized CXCL12 Antibody Delays Onset and Modulates Immune Response in Alopecia Areata Mice: Insights from Single-Cell RNA Sequencing

research Humanized CXCL12 antibody delays onset and modulates immune response in alopecia areata mice: insights from single-cell RNA sequencing

7 citations , October 2024 in “Frontiers in Immunology”

In this study, researchers prepared a humanized CXCL12 antibody for alopecia areata treatment, finding it significantly delayed disease onset in mice and reduced immune cell activation, suggesting potential as an immune modulatory therapy.

research Single‐cell RNA sequencing highlights the role of proinflammatory fibroblasts, vascular endothelial cells, and immune cells in the keloid immune microenvironment

6 citations , October 2024 in “International Journal of Dermatology”

In this study, researchers conducted single-cell RNA sequencing to reveal that proinflammatory fibroblasts and vascular endothelial cells play significant roles in the immune microenvironment of keloids, suggesting potential targets for new therapeutic approaches.

research Innovations in Skin Regeneration: The Intersection of 3D Bioprinting and Single-Cell RNA Sequencing

January 2026 in “International journal of high school research”

This review discusses how combining single-cell RNA sequencing and 3D bioprinting is advancing skin tissue engineering by enhancing cellular-level precision and addressing challenges like vascularization, ultimately improving regenerative outcomes and therapeutic strategies.

research Efficient integration of human skin single-cell RNA sequencing data

December 2025

This research by Yuan et al. focused on developing a comprehensive human skin cell atlas, analyzing various cell types and diseases, and introduced a deep learning method, scSEA, for unbiased reference mapping, potentially discovering new cell types.

research Efficient integration of human skin single-cell RNA sequencing data

December 2025

The researchers developed a comprehensive human skin cell atlas using data from various studies and established a consensus nomenclature for normal human skin in this project, which also includes a deep learning-based method for more effective reference mapping of new cells.

research 057 Single cell RNA and TCR sequencing reveals hyperexpansion of T cell clones and novel regulatory mechanisms of CD8+ T cells in murine alopcecia areata skin and draining lymph nodes

July 2024 in “Journal of Investigative Dermatology”

This study found that in mice with alopecia areata, CD8+ T cells showed clonal expansion and specific regulatory networks, which might help identify new therapeutic targets for patients not responding to JAK inhibitors.

research LB1027 Identifying the mechanism of tension-associated tissue fibrosis based on single cell RNA sequencing on keloid scar

July 2024 in “Journal of Investigative Dermatology”

Mechanical tension worsens keloid scars by activating inflammation and fibrosis pathways.

research Molecular heterogeneity of quiescent melanocyte stem cells revealed by single-cell RNA-sequencing

December 2023 in “bioRxiv (Cold Spring Harbor Laboratory)”

This research examined the transcriptional landscape of quiescent melanocyte stem cells (qMcSCs) in adult female mice, revealing significant heterogeneity within this cell population and identifying novel subpopulations that vary in immune privilege regulation, melanocyte differentiation potential, and neural crest potential.

research Integrated single-cell RNA-sequencing data of unwounded and wounded mouse skin and fibroblasts.

November 2023 in “Zenodo (CERN European Organization for Nuclear Research)”

In this study, Almet et al. (2023) compiled integrated single-cell RNA sequencing data from multiple mouse datasets to investigate how fibroblasts evolve during wound healing by examining changes in the extracellular matrix and signaling pathways.

research Integrated single-cell RNA-sequencing data of unwounded and wounded mouse skin and fibroblasts.

November 2023 in “Zenodo (CERN European Organization for Nuclear Research)”

This study does not report results but provides integrated single-cell RNA sequencing data from multiple mouse wound healing models, offering resources for studying fibroblast-driven changes in the extracellular matrix and signaling during the healing process.

research 165 Single-cell RNA sequencing analysis unveils highly active yet diminished CD14+CD16- circulating monocytes, independent of alopecia areata severity

November 2023 in “Journal of Investigative Dermatology”

Highly active but fewer CD14+CD16- monocytes are found in Alopecia Areata patients, regardless of severity.

Single-Cell RNA Sequencing and Proteomic Analysis of Dermal Fibroblasts Cultured From Female Scalp Identifies Significant Alterations in Cell Populations With Age

research O21 Single-cell RNA sequencing and proteomic analysis of dermal fibroblasts cultured from female scalp identifies significant alterations in cell populations with age

July 2023 in “British journal of dermatology/British journal of dermatology, Supplement”

This study investigated age-related changes in female scalp dermal fibroblasts, finding alterations in gene and protein expressions associated with fibrosis and senescence, which could potentially affect hair follicle health and contribute to aging-related hair changes.

Updated Skin Transcriptomic Atlas Depicted by Reciprocal Contribution of Single-Nucleus RNA Sequencing and Single-Cell RNA Sequencing

research 1347 Updated skin transcriptomic atlas depicted by reciprocal contribution of single-nucleus RNA sequencing and single-cell RNA sequencing

April 2023 in “Journal of Investigative Dermatology”

This study found that single-nucleus RNA sequencing identified more relevant keratinocyte clusters and specific markers than single-cell RNA sequencing, offering a new perspective on skin cell differentiation and function.

research 1410 Single-cell RNA sequencing reveals POUF51 and HES3 as regulators of the stem cell population in psoriasis

April 2023 in “Journal of Investigative Dermatology”

This research reexamined transcriptomic data to study stem and progenitor cell proliferation in psoriasis, finding that the number of committed progenitor cells increased eight-fold in psoriatic skin without altering stem cell numbers, potentially identifying new therapeutic targets.

Single-Cell RNA Sequencing of Different Fibroblast Populations Cultured from Female Scalp Reveals Significant Alterations to Transcriptional Profiles with Age

research 1424 Single-cell RNA sequencing of different fibroblast populations cultured from female scalp reveals significant alterations to transciptional profiles with age

April 2023 in “The journal of investigative dermatology/Journal of investigative dermatology”

This study identified distinct and diverse fibroblast populations in female scalp cells that lose their signature and identity with age, highlighting significant age-related changes, such as increased fibrosis, DNA damage, and senescence, which may affect scalp dermal support for healthy hair follicles.