Molecular and Spatial Design of Early Skin Development

December 2022 in “ bioRxiv (Cold Spring Harbor Laboratory) ”

Tina Jacob, Karl Annusver, Paulo Czarnewski, Tim Dalessandri, Maria Eleni Kastriti, Chiara Levra Levron, Marja L. Mikkola, Michael Rendl, Beate M. Lichtenberger, Giacomo Donati, Åsa K. Björklund, Maria Kasper

hair follicle induction fibroblasts panniculus carnosus muscle epithelial heterogeneity periderm layer mast cells dendritic immune cells

TLDR The research reveals how early embryonic mouse skin develops from simple to complex structures, identifying various cell types and their roles in this process.

This study uses single-cell transcriptomics, spatial cell-type assignment, and cell-lineage tracing to investigate the development of early embryonic mouse skin. The research uncovers the progression from uniform developmental precursors to a complex, multilayered epithelium with diverse cell types and tissue architecture. Key findings include the identification of fibroblasts involved in hair follicle induction, muscle support, and fascia formation, as well as the early formation of the panniculus carnosus muscle, blood vessels lacking pericyte coverage, and the initial presence of mast and dendritic immune cells in the skin. Additionally, the study reveals unexpected epithelial heterogeneity within the single-layered epidermis and a signaling-rich periderm layer. This cellular blueprint of skin development provides important histological landmarks and insights into the dynamic interactions among skin cells during early development.

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