Porcine Acellular Dermal Matrix Facilitates Hair Follicle Stem Cells Entering the Hair Cycle in C57/BL6 Mice

The study investigated the use of a porcine collagen acellular dermal matrix (ADM) scaffold, developed using supercritical carbon dioxide extraction technology, for hair regeneration in C57BL/6 mice. The ADM scaffold, when combined with mice Platelet-Rich Plasma (PRP), showed a synergistic effect on hair growth. Subdermal injection of the ADM scaffold led to the modulation of CD34-positive stem cells and the expression of hair cycle differentiation markers such as Sox9, Wnt3a, β-catenin, Ki67, and Lef-1, resulting in increased hair follicles and hair density. The findings suggest that the SCCO2-derived porcine ADM scaffold could be a potential therapeutic solution for alopecia, with no observed adverse effects.