Simultaneous Pharmacokinetic and Pharmacodynamic Analysis of 5α-Reductase Inhibitors and Androgens by Liquid Chromatography Tandem Mass Spectrometry

August 2014 in “ Talanta ”

Rita Upreti, Gregorio Naredo, Abdullah M.M. Faqehi, Katherine A. Hughes, Laurence Stewart, Brian R. Walker, Natalie Homer, Ruth Andrew

5α-reductase inhibitors finasteride dutasteride DHT androgens liquid chromatography tandem mass spectrometry Propecia Avodart

TLDR The method effectively measures drug impact on DHT levels in prostate treatments.

The study developed a novel assay using liquid chromatography tandem mass spectrometry (LC-MS/MS) to measure the concentrations of 5α-reductase inhibitors (finasteride and dutasteride), their substrates, and the product (DHT) in human serum. Tested on 16 male subjects aged 20-85 years, the method demonstrated precision, accuracy, and stability, requiring only 500 µL of serum. Both finasteride and dutasteride significantly suppressed DHT concentrations by approximately 47% while increasing testosterone levels. The assay provided a robust marker of treatment adherence and effectiveness, although further technological advances were suggested to enhance its sensitivity and measurable pharmacodynamic range.

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Research cited in this study

18 / 18 results

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Related Community Posts Join

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