Picogram Determination of Finasteride in Human Plasma and Semen by High-Performance Liquid Chromatography with Atmospheric-Pressure Chemical-Ionization Tandem Mass Spectrometry

    M.L Constanzer, C.M. Chavez, Bogdan J. Matuszewski
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    TLDR Method detects finasteride in plasma and semen with high sensitivity and accuracy.
    This document describes a method for detecting the drug finasteride in human plasma and semen using high-performance liquid chromatography with atmospheric-pressure chemical-ionization tandem mass spectrometry. The method was validated in both plasma and semen, with a high sensitivity of detection allowing for an assay with a limit of quantification of 200 pg per ml of biological fluid. The within-day precision of the assay was less than 10% at all concentrations within the standard curve range, and the assay accuracy at all concentrations both in plasma and semen varied between 94-110%. The recovery of finasteride was ≥ 90% at all concentrations within the standard curve range and was practically the same from plasma and semen.
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