TLDR Method detects finasteride in plasma and semen with high sensitivity and accuracy.
This document describes a method for detecting the drug finasteride in human plasma and semen using high-performance liquid chromatography with atmospheric-pressure chemical-ionization tandem mass spectrometry. The method was validated in both plasma and semen, with a high sensitivity of detection allowing for an assay with a limit of quantification of 200 pg per ml of biological fluid. The within-day precision of the assay was less than 10% at all concentrations within the standard curve range, and the assay accuracy at all concentrations both in plasma and semen varied between 94-110%. The recovery of finasteride was ≥ 90% at all concentrations within the standard curve range and was practically the same from plasma and semen.
19 citations,
May 1991 in “Journal of Chromatography B: Biomedical Sciences and Applications” Reliable method detects finasteride in human plasma at low doses.
211 citations,
November 1990 in “The Journal of Steroid Biochemistry and Molecular Biology” Finasteride effectively treats BPH, but needs more trials to understand potential.
193 citations,
August 1985 in “Endocrinology” Different animals have unique versions of the enzyme that changes testosterone into another hormone, which is important for creating effective treatments for prostate and hair loss conditions.
18 citations,
July 2009 in “Drug Metabolism and Disposition” Finasteride breakdown products found in bile and urine, helps understand drug safety and effectiveness.
3 citations,
February 2021 in “Molecules” A new method was created to test the effectiveness of Dihydrotestosterone (DHT) inhibitors, like finasteride and dutasteride, in human and fish cells. The results showed fish cells are more sensitive to these treatments, and dutasteride works better than finasteride in all tested cells.
1 citations,
April 2015 in “Drug research” New method measures finasteride in plasma, finds two formulations bioequivalent.
4 citations,
March 2019 in “Acta Chromatographica” Two methods can measure finasteride and tamsulosin hydrochloride; HPLC is more advantageous, while TLC offers better separation.
13 citations,
May 2006 in “Analytical Biochemistry” New method accurately measures finasteride in tablets.
