Development of a Liquid Chromatography/Mass Spectrometry-Based Inhibition Assay for Screening Steroid 5-Alpha Reductase in Human and Fish Cell Lines

    February 2021 in “ Molecules ”
    Dahye Kim, Hyun-Ki Cho, Ruth Eggers … Young Ho Kim
    Studysummary In this study, researchers developed a sensitive assay to measure the inhibition of steroid 5-α reductase (5AR) by chemicals like finasteride and dutasteride, revealing species-specific differences in inhibitor efficacy between human and fish cell lines.
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    Research cited in this study 11

    1. Role of 5α-Dihydrotestosterone in Testicular Development of Gilthead Seabream Following Finasteride Administration The Journal of Steroid Biochemistry and Molecular Biology · 2017
    2. Bioactives in Chinese Proprietary Medicine Modulate 5α-Reductase Activity and Gene Expression Associated with Androgenetic Alopecia Frontiers in Pharmacology · 2017
    3. A New Label-Free Screen for Steroid 5α-Reductase Inhibitors Using LC-MS Steroids · 2016
    4. Identification of a New Plant Extract for Androgenic Alopecia Treatment Using a Non-Radioactive Human Hair Dermal Papilla Cell-Based Assay BMC Complementary and Alternative Medicine · 2015
    5. Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus Acutissima Cortex Evidence-based Complementary and Alternative Medicine · 2015
    6. Determination Method for Steroid 5α-Reductase Activity Using Liquid Chromatography/Atmospheric Pressure Chemical Ionization–Mass Spectrometry Analytical Sciences · 2005
    7. The Long-Term Effect of Doxazosin, Finasteride, and Combination Therapy on the Clinical Progression of Benign Prostatic Hyperplasia The New England Journal of Medicine · 2003
    8. PNU 157706, A Novel Dual Type I and II 5α-Reductase Inhibitor The Journal of Steroid Biochemistry and Molecular Biology · 1998
    9. Inhibition of Rat Alpha-Reductases by Finasteride: Evidence for Isozyme Differences in the Mechanism of Inhibition The Journal of Steroid Biochemistry and Molecular Biology · 1997
    10. Rat Steroid 5α-Reductase Kinetic Characteristics: Extreme pH-Dependency of the Type II Isozyme in Prostate and Epididymis Homogenates The Journal of Steroid Biochemistry and Molecular Biology · 1995
    11. Hair Growth Effects of Oral Administration of Finasteride, a Steroid 5 Alpha-Reductase Inhibitor, Alone and in Combination with Topical Minoxidil in the Balding Stumptail Macaque The Journal of Clinical Endocrinology and Metabolism · 1992

    Related research 2

    1. Finasteride Inhibits 5α-Reductase Activity in Human Dermal Fibroblasts: Prediction of Its Therapeutic Application in Androgen-Related Skin Diseases International Journal of Dermatology · 1995
    2. Effect of Finasteride, a 5 Alpha-Reductase Inhibitor, on Serum Gonadotropins in Normal Men The Journal of Clinical Endocrinology and Metabolism · 1992