Development of a Liquid Chromatography/Mass Spectrometry-Based Inhibition Assay for Screening Steroid 5-Alpha Reductase in Human and Fish Cell Lines

February 2021 in “ Molecules ”

Dahye Kim, Hyun-Ki Cho, Ruth Eggers, Sang Hoon Kim, Chang Y. Ryu, Young Ho Kim

Dihydrotestosterone DHT 5-alpha reductase 5AR finasteride dutasteride testosterone steroid metabolism endocrine disruption Propecia Avodart

From the encyclopedia

Finasteride →

Frontline, gold standard treatment for combatting androgenic alopecia

Dutasteride →

Heavy duty finasteride that comes with higher risks

Studysummary In this study, researchers developed a sensitive assay to measure the inhibition of steroid 5-α reductase (5AR) by chemicals like finasteride and dutasteride, revealing species-specific differences in inhibitor efficacy between human and fish cell lines.

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research Identification of a New Plant Extract for Androgenic Alopecia Treatment Using a Non-Radioactive Human Hair Dermal Papilla Cell-Based Assay

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This study found that Avicennia marina extract significantly inhibited 5α-reductase type 1 activity in human hair dermal papilla cells, suggesting its potential use for treating androgenic alopecia.

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research Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus Acutissima Cortex

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research Determination Method for Steroid 5α-Reductase Activity Using Liquid Chromatography/Atmospheric Pressure Chemical Ionization–Mass Spectrometry

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This study developed a method using LC/APCI-MS to measure the activity of steroid 5α-reductase, applied to kinetic studies and inhibitory tests with Finasteride in a rat prostatic enzyme model.

research The Long-Term Effect of Doxazosin, Finasteride, and Combination Therapy on the Clinical Progression of Benign Prostatic Hyperplasia

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research PNU 157706, A Novel Dual Type I and II 5α-Reductase Inhibitor

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research Inhibition of Rat Alpha-Reductases by Finasteride: Evidence for Isozyme Differences in the Mechanism of Inhibition

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This study found that the pH dependency of rat steroid 5α-reductase type II isozyme significantly affects its kinetic properties, including Vmax and Km, suggesting past discrepancies in literature may arise from these pH variances during assays.

Hair Growth Effects of Oral Administration of Finasteride, a Steroid 5 Alpha-Reductase Inhibitor, Alone and in Combination with Topical Minoxidil in the Balding Stumptail Macaque

research Hair Growth Effects of Oral Administration of Finasteride, a Steroid 5 Alpha-Reductase Inhibitor, Alone and in Combination with Topical Minoxidil in the Balding Stumptail Macaque

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In this study, the combination of finasteride and minoxidil significantly increased hair growth in balding male stumptail macaques compared to each drug alone.