A New Label-Free Screen for Steroid 5α-Reductase Inhibitors Using LC-MS

December 2016 in “ Steroids ”

Jukkarin Srivilai, Karma Rabgay, Nantaka Khorana, Neti Waranuch, Nitra Nuengchamnong, Kornkanok Ingkaninan

steroid 5α-reductase LC-MS androgenic alopecia Impatiens balsamina Curcuma longa S5aR anti-androgenic compounds liquid chromatography-mass spectrometry S5aR inhibitors hair loss herbal extracts

TLDR Researchers developed a new method to find substances in herbs that can block a specific enzyme linked to hair loss.

In 2016, researchers developed a label-free liquid chromatography-mass spectrometry (LC-MS) assay to identify inhibitors of steroid 5α-reductase (S5aR), an enzyme linked to conditions like androgenic alopecia. The assay was optimized and validated, showing high reproducibility with a Z' factor of 0.77. It was applied to screen 11 Thai herbal extracts, with Impatiens balsamina L. and Curcuma longa L. demonstrating significant S5aR inhibitory activity, with IC50 values of 5.4±0.2 µg/mL and 9.0±1.2 µg/mL, respectively. The study concluded that the assay is suitable for medium throughput screening and could be used in small laboratory settings to identify potential anti-androgenic compounds in herbal extracts.

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