Measurement of Tamsulosin in Human Serum by Liquid Chromatography–Tandem Mass Spectrometry

A method was developed and validated for extracting and quantifying tamsulosin from human serum using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The process involved liquid-liquid extraction with methyl tert-butyl ether and achieved 99.9% analyte recovery. The method used an Ascentis Express C18 column and a triple quadrupole mass spectrometer, with a total run-time of 6 minutes. It demonstrated a limit of quantitation of 0.2 ng/mL and was validated for a linear range of 0.2-50 ng/mL, showing acceptable precision, accuracy, and stability. This method was applied in a clinical study on benign prostatic hyperplasia, serving as a measure of compliance in clinical research.