RP-HPLC Assay Method for Simultaneous Estimation of Tamsulosin Hydrochloride and Finasteride in Pharmaceutical Dosage Form

A Reverse Phase High Performance Liquid Chromatographic method was developed for the simultaneous estimation of tamsulosin hydrochloride and finasteride in tablet form. The method utilized an RP C18 BDS column with a methanol-water mobile phase and UV detection at 260 nm. Retention times were 2.68 min for tamsulosin hydrochloride and 7.33 min for finasteride. The method was validated for various parameters, showing high precision and accuracy, with linear calibration curves for both compounds. The detection limits were 0.28 µg/ml for tamsulosin hydrochloride and 0.85 µg/ml for finasteride, while quantitation limits were 1.03 µg/ml and 3.22 µg/ml, respectively. Recovery studies indicated average recoveries of 99.9%-100.54% for tamsulosin hydrochloride and 99.83%-100.92% for finasteride, with % RSD below 2.0, confirming the method's precision and reliability.