Rapid Determination of Finasteride in Human Plasma by UPLC-MS/MS and Its Application to Clinical Pharmacokinetic Study

The paper describes a validated UPLC-MS/MS method for quantifying finasteride in human plasma, with a linear response from 0.1 to 30 ng/mL and a limit of quantitation of 0.1 ng/mL. The method was successfully applied to a pharmacokinetic study of finasteride in healthy volunteers following oral administration. The UPLC-MS/MS method described herein boasts significant advantages over other techniques, including LC-MS/MS, due to the inherently increased column efficiency of UPLC, which resulted in complete analysis within 3.5 min with significantly lower limits of quantitation (0.1 ng/mL).