Rapid and Sensitive LC-MS-MS Method for the Simultaneous Estimation of Alfuzosin and Dutasteride in Human Plasma

The study developed and validated a liquid chromatography–tandem mass spectrometric method for estimating alfuzosin and dutasteride in human plasma. The method used solid-phase extraction with terazosin and finasteride as internal standards, achieving chromatographic separation on a Hypurity C18 column. Detection was performed using an API 5000 mass spectrometer, allowing for rapid analysis with a total run time of 2.5 minutes. The method was validated for alfuzosin and dutasteride over specific concentration ranges, showing consistent and reproducible recoveries. It demonstrated acceptable precision and accuracy, enabling the analysis of over 180 plasma samples per day. This method was applicable for pharmacokinetic, bioavailability, or bioequivalence studies, as illustrated by its use in a bioequivalence study.