Determination Method for Steroid 5α-Reductase Activity Using Liquid Chromatography/Atmospheric Pressure Chemical Ionization–Mass Spectrometry

October 2005 in “ Analytical Sciences ”

Kuniko Mitamura, Chie Ogasawara, Asami Shiozawa, Erika Terayama, Kazutake Shimada

steroid 5α-reductase 5α-dihydrotestosterone 5α-androstane-3α,17β-diol Finasteride liquid chromatography atmospheric pressure chemical ionization mass spectrometry Propecia

TLDR A new method was developed to measure and assess the activity and inhibition of the enzyme steroid 5α-reductase.

The study developed a method to determine steroid 5α-reductase activity using liquid chromatography/atmospheric pressure chemical ionization–mass spectrometry (LC/APCI-MS) in positive-ion mode. The method utilized a rat prostatic enzyme source to measure the enzymatic formation of 5α-dihydrotestosterone and 5α-androstane-3α,17β-diol, with the sum of these products indicating enzyme activity. This approach was applied to kinetic studies and inhibitory experiments using Finasteride® as a model inhibitor, providing a means to assess the enzyme's activity and inhibition.

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Research cited in this study

3 / 3 results

research Dihydrotestosterone and the Concept of 5-Alpha-Reductase Inhibition in Human Benign Prostatic Hyperplasia

184 citations , January 2000 in “European Urology”

Blocking the enzyme that turns testosterone into DHT can safely and effectively treat enlarged prostate.

research FCE 28260, A New 5α-Reductase Inhibitor: In Vitro and In Vivo Effects

17 citations , June 1996 in “The Journal of Steroid Biochemistry and Molecular Biology”

FCE 28260 is a stronger and longer-lasting inhibitor of 5α-reductase than finasteride, which may make it a better treatment for certain medical conditions.

research Species Differences in Prostatic Steroid 5α-Reductases of Rat, Dog, and Human

193 citations , August 1985 in “Endocrinology”

Different animals have unique versions of the enzyme that changes testosterone into another hormone, which is important for creating effective treatments for prostate and hair loss conditions.

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community Finasteride does deplete important nuero-active steroids

in Research/Science 9 upvotes 2 years ago

Finasteride can reduce neuroactive steroids, causing side effects like depression, anxiety, and sexual dysfunction. Some users experience persistent symptoms after stopping finasteride, while others use alternative treatments like topical solutions.

community 28M experiencing thinning - first timer here, please help

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A 28-year-old male experiencing hair thinning is considering minoxidil and finasteride. Minoxidil helps regrow hair, while finasteride prevents further loss; both are usually lifelong treatments.

community Can I still save my hairline at age of 15?

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A 15-year-old is concerned about hair loss, possibly at Norwood 2 or 3, and is using shampoos and conditioners recommended by a trichologist. Suggestions include considering topical minoxidil and consulting a doctor about topical anti-DHT treatments like finasteride or RU58841, but avoiding 5-alpha-reductase inhibitors at this age.

community Finasteride only reduces scalp DHT by ~40%. Why does it work?

in Finasteride/Dutasteride 37 upvotes 2 years ago

How Finasteride and Dutasteride help reduce hair loss by reducing the amount of DHT in scalp areas, as well as discussing factors such as sensitivity to DHT, 5α-reductase expression in different parts of the hair follicle, and scalp tension.

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in Research/Science 140 upvotes 8 months ago

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in Research/Science 106 upvotes 4 years ago

Finasteride was intentionally developed to treat BPH and later approved for male pattern baldness (MPB) due to its 5AR inhibition effects. The delay in MPB approval was due to concerns about off-label use for female hirsutism and the prioritization of treating a more debilitating condition.

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