Determination Method for Steroid 5α-Reductase Activity Using Liquid Chromatography/Atmospheric Pressure Chemical Ionization–Mass Spectrometry

October 2005 in “ Analytical Sciences ”

Kuniko Mitamura, Chie Ogasawara, Asami Shiozawa, Erika Terayama, Kazutake Shimada

steroid 5α-reductase 5α-dihydrotestosterone 5α-androstane-3α,17β-diol Finasteride liquid chromatography atmospheric pressure chemical ionization mass spectrometry Propecia

TLDR A new method was developed to measure and assess the activity and inhibition of the enzyme steroid 5α-reductase.

The study developed a method to determine steroid 5α-reductase activity using liquid chromatography/atmospheric pressure chemical ionization–mass spectrometry (LC/APCI-MS) in positive-ion mode. The method utilized a rat prostatic enzyme source to measure the enzymatic formation of 5α-dihydrotestosterone and 5α-androstane-3α,17β-diol, with the sum of these products indicating enzyme activity. This approach was applied to kinetic studies and inhibitory experiments using Finasteride® as a model inhibitor, providing a means to assess the enzyme's activity and inhibition.

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