Determination Method for Steroid 5α-Reductase Activity Using Liquid Chromatography/Atmospheric Pressure Chemical Ionization–Mass Spectrometry
October 2005
in “
Analytical Sciences
”
The study developed a method to determine steroid 5α-reductase activity using liquid chromatography/atmospheric pressure chemical ionization–mass spectrometry (LC/APCI-MS) in positive-ion mode. The method utilized a rat prostatic enzyme source to measure the enzymatic formation of 5α-dihydrotestosterone and 5α-androstane-3α,17β-diol, with the sum of these products indicating enzyme activity. This approach was applied to kinetic studies and inhibitory experiments using Finasteride® as a model inhibitor, providing a means to assess the enzyme's activity and inhibition.
