Establishment and Characterization of Matched Immortalized Human Frontal and Occipital Scalp Dermal Papilla Cell Lines from Androgenetic Alopecia

December 2023 in “ Scientific Reports ”

Mi Hee Kwack, Ons Ben Hamida, Min Kyu Kim, Moon Kyu Kim, Young Kwan Sung

androgenetic alopecia dihydrotestosterone DHT androgen receptor AR 5-alpha reductase type 2 5αR2 hair follicle miniaturization hair loss dermal papilla cells SV40T-Ag hTERT hair growth factors AGA

Studysummary In this study, researchers successfully established immortalized human frontal and occipital scalp dermal papilla cell lines from androgenetic alopecia patients, observing distinct gene expression, androgen receptor levels, and hair follicle growth effects between these regions, which may help advance hair loss research and therapeutic development.

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research TERT/BMI1-Transgenic Human Dermal Papilla Cells Enhance Murine Hair Follicle Formation In Vivo

1 citations , March 2022 in “Journal of Dermatological Science”

This study concluded that overexpressing TERT and BMI1 in cultured human dermal papilla cells extended their lifespan and enhanced their ability to induce hair growth in mice.

research Expression Level of Leucine-Rich Repeat Containing 15 Regulates Characteristics of Dermal Papilla Cells of Human Hair Follicle

1 citations , September 2020 in “Journal of Dermatological Science”

In this study, researchers found that the gene LRRC15 was overexpressed in dermal papilla cells from balding areas compared to non-balding areas in patients with androgenetic alopecia.

research HB-EGF Improves the Hair Regenerative Potential of Adipose-Derived Stem Cells via ROS Generation and Hck Phosphorylation

20 citations , December 2019 in “International Journal of Molecular Sciences”

This study found that preconditioning adipose-derived stem cells with HB-EGF enhanced their ability to promote hair growth in vivo by increasing their motility, paracrine effects, and survival.

research Overexpression of Alkaline Phosphatase Improves the Hair-Inductive Capacity of Cultured Human Dermal Papilla Spheres

13 citations , July 2019 in “Journal of Dermatological Science”

This study suggests that 3D spheroid cultivation of dermal papilla cells can restore their hair-inductive capabilities, which are lost in 2D-cultured cells.

research A Role for the TGF-β/BMP Co-Receptor Endoglin in the Molecular Oscillator That Regulates the Hair Follicle Cycle

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This study observed that Endoglin is crucial for maintaining correct hair follicle cycling and stimulating hair follicle stem cell niches through feedback interactions involving Wnt/β-catenin and Bmp/Smad signaling pathways in a mouse model.

research Establishment and Characterization of Five Immortalized Human Scalp Dermal Papilla Cell Lines

16 citations , January 2018 in “Biochemical and Biophysical Research Communications”

In this study, researchers established five new immortalized human dermal papilla cell lines from a male with androgenetic alopecia, which may serve as valuable tools for hair research.

research SFRP2 Augments Wnt/β-Catenin Signalling in Cultured Dermal Papilla Cells

17 citations , February 2016 in “Experimental Dermatology”

This study found that SFRP2 enhances Wnt3a-mediated β-catenin signaling in human dermal papilla cells, with higher SFRP2 expression in beard cells correlating with increased trichogenicity.

research Fibroblast Growth Factors Stimulate Hair Growth Through Beta-Catenin and Shh Expression in C57BL/6 Mice

64 citations , January 2015 in “BioMed Research International”

In this study, topical application of fibroblast growth factors was found to promote hair growth by inducing and prolonging the anagen phase in telogenic C57BL/6 mice, suggesting potential as hair growth-promoting agents.

research Differentiation of Human Hair Follicle Stem Cells Into Endothelial Cells Induced by Vascular Endothelial and Basic Fibroblast Growth Factors

24 citations , November 2013 in “Molecular Medicine Reports”

This study demonstrated that human hair follicle stem cells can be efficiently differentiated into endothelial-like cells using VEGF and bFGF, suggesting their potential as a novel cell source for vascular tissue engineering.

Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

research Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

256 citations , October 2013 in “Proceedings of the National Academy of Sciences of the United States of America”

This study found that altering cell culture conditions to form three-dimensional papilla spheroids can restore the ability of human dermal papilla cells to induce hair growth in adult skin.

research Strategies to Enhance Epithelial-Mesenchymal Interactions for Human Hair Follicle Bioengineering

57 citations , February 2013 in “Journal of Dermatological Science”

This article reviews methods to enhance epithelial–mesenchymal interactions for hair follicle bioengineering but does not present new experimental results; it emphasizes optimizing combinations for successful regeneration.

Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay

research Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay

75 citations , August 2011 in “Journal of Investigative Dermatology”

This study found that cultured human dermal papilla cells formed into three-dimensional spheres have an increased ability to induce hair follicles from mouse epidermal cells compared to two-dimensional cultures.

research Molecular Basis of Androgenetic Alopecia: From Androgen to Paracrine Mediators Through Dermal Papilla

122 citations , November 2010 in “Journal of Dermatological Science”

This study found that increased expression of type II 5α-reductase, androgen receptors, and Hic-5/ARA55 in dermal papilla cells upregulates androgen sensitivity, playing a key role in androgenetic alopecia pathogenesis.

research Review of Hair Follicle Dermal Cells

321 citations , December 2009 in “Journal of Dermatological Science”

This review discusses the role of dermal cells in hair follicle regeneration and morphogenesis and highlights their potential therapeutic implications, but it reports no new empirical findings.

research The Mesenchymal Component of Hair Follicle Neogenesis: Background, Methods, and Molecular Characterization

131 citations , July 2009 in “Experimental Dermatology”

This review discusses the development and advances in studying trichogenic dermal cells for hair follicle morphogenesis, summarizing methods, bioassays, and molecular markers, but reports no new research findings.

research Sox2-Positive Dermal Papilla Cells Specify Hair Follicle Type in Mammalian Epidermis

338 citations , July 2009 in “Development”

This study demonstrates that Sox2-positive dermal papilla cells specify particular hair follicle types by showing heterogeneity in gene expression and pathway activation in these cells.

research Androgens and Hair Growth

209 citations , September 2008 in “Dermatologic Therapy”

This review discusses the role of androgens in hair follicle regulation and suggests that a better understanding could enhance treatments for conditions like hirsutism and alopecia, but does not present new findings.

research Premature Senescence of Balding Dermal Papilla Cells In Vitro Is Associated With p16INK4a Expression

141 citations , November 2007 in “Journal of Investigative Dermatology”

This study found that balding dermal papilla cells show premature senescence and altered expression of stress and DNA damage markers, suggesting sensitivity to environmental stress in androgenetic alopecia.

research Hormonal Regulation of Hair Follicles Exhibits a Biological Paradox

98 citations , February 2007 in “Seminars in Cell & Developmental Biology”

This review explores the hormonal regulation of hair growth and changes with season, age, and sexual development, and calls for improved treatments for hair disorders.

research Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle

417 citations , September 2005 in “PLoS biology”

This study developed molecular signatures for dermal papilla cells and their niche, uncovering novel signaling regulators and genes linked to hair disorders, which may inform future hair development research.

research Role of Androgen in Mesenchymal Epithelial Interactions in Human Hair Follicle

67 citations , August 2005 in “Journal of Investigative Dermatology Symposium Proceedings”

This study found that in vitro, beard dermal papilla cells stimulated keratinocyte proliferation via androgen-dependent growth factors, while androgenetic alopecia cells released TGF-β1, inhibiting keratinocyte growth.

Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla

research Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla

208 citations , December 2003 in “Journal of Investigative Dermatology”

This study found that dermal papilla and peribulbar dermal sheath cup cells in mice can both induce hair follicle formation, suggesting they may have similar functional roles in hair growth.

research Molecular Control of Epithelial-Mesenchymal Interactions During Hair Follicle Cycling

277 citations , June 2003 in “The journal of investigative dermatology. Symposium proceedings/The Journal of investigative dermatology symposium proceedings”

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research Molecular Genetic and Endocrine Mechanisms of Hair Growth

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research Molecular Mechanisms of Androgenetic Alopecia

229 citations , August 2002 in “Experimental Gerontology”

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research Endogenous Production of Heparin-Binding EGF-Like Growth Factor During Murine Partial-Thickness Burn Wound Healing

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research Molecular Mechanisms Regulating Hair Follicle Development

854 citations , February 2002 in “The journal of investigative dermatology/Journal of investigative dermatology”

This review summarizes recent advances in understanding the molecular mechanisms of hair follicle formation and discusses potential future clinical applications for treating hair loss and skin tumors, but it reports no new clinical results.

research Cellular And Developmental Aspects Of Androgenetic Alopecia

192 citations , October 1998 in “Experimental Dermatology”

This article examines the terminal to vellus transition in androgenetic alopecia through cellular and developmental mechanisms, discussing challenges and knowledge gaps without presenting new clinical findings.