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This study found that using 3D cultures and fetal tissue extracts significantly improved the outcomes of stem cell treatments for regenerative medicine, including hair follicle formation and chronic wound healing.

This review discusses various approaches to treating alopecia, including drug therapy and hair transplantation, but reports no new research findings; the authors highlight the need for novel techniques like cell culturing.

This review discusses strategies for hair follicle regeneration using tissue engineering and reports no new clinical results; the authors highlight potential future directions for promoting hair neoformation.

This review discusses the development of hair-on-a-chip technology for hair follicle research and alopecia treatment, reporting no new clinical results but highlighting future research directions.

This study reports that incorporating Lef-1 transfected dermal papilla cells into human skin equivalents significantly promoted hair follicle differentiation and hair fiber growth, offering a potential advancement for managing skin loss.

This study observed that a 3D culture model of hair follicle cells showed an anagen-like phase between days 3 to 6 and transitioned to a catagen-like phase by day 7, highlighting cell differentiation and structural development over time.

This study introduced a reproducible human model using 3D-SeboSkin technology to study hidradenitis suppurativa, allowing better maintenance of skin integrity and replication of biomarker expression patterns compared to traditional skin cultures, suggesting its value for further research.

This project report reviews six experimental 3D bioprinting methods for cultivating artificial hair follicles, noting significant challenges such as method complexity, low output, and delayed approval for human trials, while proposing a potential solution for creating a permanent hair system on the scalp.

This study found that a novel 3D in vitro culture condition improved stemness markers and proliferation potential in human dermal stem/progenitor cells compared to traditional 2D methods.

This study found that a titrated extract of Centella asiatica enhanced the hair growth potential of 3D spheroid cultured human dermal papilla cells.

This study found that anisotropic osmolyte solutions may improve hair follicle turnover and condition in both in vitro and in silico models, as well as in human volunteers with hair disorders.

This study proposes a two-step method that may enhance the generation of stem-like epidermal cells with superior proliferation and differentiation potential for skin regeneration.

In this study, researchers demonstrated that using 3D spheroid formation and genetic modification significantly enhanced the hair-inducing capacity of human dermal papilla cells in engineered skin constructs.

This study investigated the use of 3D cell culture technology to explore hair follicle regeneration, examining the impact of the 3D cellular environment on hair follicle morphogenesis and the effects of microwell depth on spheroid formation.

This review discusses recent advancements in regenerative medicine, including functional organ regeneration and the creation of mini-organs, and reports no new clinical results.

This review discusses the potential of adipose-derived stem cells for treating vitiligo, hair loss, and chronic wound healing, but reports no new clinical results and suggests the need for further investigation.

This review discusses the advancements and challenges in developing in vitro human skin models incorporating components like vasculature for drug testing and disease research, but reports no new clinical results.

This review highlights recent advancements in microfluidics for 3D tissue model generation and discusses its applications in tissue engineering and high-throughput drug screening without reporting new experimental results.

Innovative methods are reducing animal testing and improving biomedical research.

This overview outlines the latest advancements and challenges in developing three-dimensional scaffolds for biomanufacturing cultured meat, focusing on organizing muscle stem cells to better mimic the structure of traditional meat. Results are not reported in the abstract.

This review highlights the development and assessment of human-relevant models for cutaneous wound healing, emphasizing that a combination of systems, such as organoid platforms and skin-on-a-chip systems, is necessary to overcome limitations of traditional methodologies and address chronic wound challenges.

This review outlines recent advances in stem cell and tissue engineering technologies in regenerative medicine and wildlife conservation, reporting no new clinical results.

This review highlights the current progress and application pros and cons of various cell-based screening platforms in drug discovery, discussing challenges and potential improvements for these innovative methods.

This study demonstrated that using a perfusion culture bioreactor and 3D spheroid culture can enhance corneal endothelial cell expansion and support the construction of tissue-engineered corneal endothelial layers in vitro.

This study identified ten key hub genes and pathways crucial for understanding the molecular mechanism of hair growth by comparing dermal papilla cells in 2D and 3D cultures.

This study found that tdDPC-EVs significantly improved wound healing by enhancing angiogenesis through the KLF4/VEGFA axis, offering advantages over traditional DPC-EVs.

This study found that co-culturing dermal papilla cells in a 3D structure with adipose-derived stem cells may enhance the expression of hair inductivity markers compared to 2D cultures.

This study found that a 3D Matrigel culture technique for dermal papilla cell spheroids can enhance hair follicle inductivity and induce hair-like fiber differentiation in vitro, even with high-passage cells.

This study found that 3D cultured hair follicle-like constructs, which include keratinocytes and dermal papilla cells, showed improved gene expression and cell interactions relevant to hair follicle development.

This study developed thermo-reversible glycol chitosan hydrogels that effectively form and maintain 3D cell spheroids within one day, offering a simplified method for creating biologically realistic cultures for tissue regeneration and drug screening applications.