Therapeutic Effect of a Xeno-Free Three-Dimensional Stem Cell Mass in a Hind Limb Ischemia Model
September 2018
in “
Tissue Engineering Part A
”
xeno-free three-dimensional stem cell masses XF-3DCMs human adipose stem cells hASCs fetal bovine serum-derived 3DCMs FBS-3DCMs pro-angiogenic factor angiogenesis markers neovascularization tissue regeneration tumorigenicity immunogenicity critical limb ischemia peripheral artery disease stem cell therapy adipose stem cells fetal bovine serum angiogenesis blood vessel growth tissue repair tumor formation immune response limb ischemia artery disease
TLDR Xeno-free three-dimensional stem cell masses are safe and effective for improving blood flow and tissue repair in limb ischemia.
The study evaluated the therapeutic potential and safety of xeno-free three-dimensional stem cell masses (XF-3DCMs) derived from human adipose stem cells (hASCs) in a mouse model of hind limb ischemia. The XF-3DCMs were compared to fetal bovine serum-derived 3DCMs (FBS-3DCMs) and showed similar or better pro-angiogenic factor secretion, significantly improved limb salvage, blood perfusion, and increased expression of angiogenesis markers without inducing tumor formation or toxicological side effects. The XF-3DCMs promoted neovascularization and tissue regeneration, with no evidence of tumorigenicity or immunogenicity, suggesting they are safe and effective for clinical applications in critical limb ischemia. The study concluded that XF-3DCMs have therapeutic effects in ischemic tissue and could be considered for clinical trials for peripheral artery disease. The specific number of mice used in the study was not provided in the summary.
