Mesenchymal Stem Cells Paracrine Proteins From Three-Dimensional Dynamic Culture System Promoted Wound Healing in Third-Degree Burn Models
July 2023
in “
Bioengineering & translational medicine
”
mesenchymal stem cell paracrine proteins 3D dynamic culture system PEG thermogel dermal fibroblasts keratinocytes re-epithelialization skin appendage recovery angiogenesis CD26+/Sca-1+ cells Dlk-1+/Sca-1+ cells MSC-PP 3D culture PEG gel fibroblasts skin cells new skin formation blood vessel formation
Studysummary This study explored a cell-free therapy with mesenchymal stem cell paracrine proteins and PEG hydrogels for deep burn treatment and found that the combination significantly enhanced wound healing, reduced scar formation, and promoted skin appendage regeneration in a rabbit model.
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The study demonstrated that mesenchymal stem cell paracrine proteins (MSC-PP) derived from a 3D dynamic culture system, combined with PEG thermogel, significantly improved wound healing in third-degree burn models. The MSC-PP treatment enhanced the migration and proliferation of dermal fibroblasts and keratinocytes, reduced inflammation, and promoted re-epithelialization, skin appendage recovery, and angiogenesis. The PEG thermogel provided a stable release of MSC-PP and exhibited no cytotoxicity, making it an effective carrier for MSC-PP delivery. The MSC-PP + PEG group showed the most effective wound contraction, minimal scarring, and the highest presence of CD26+/Sca-1+ and Dlk-1+/Sca-1+ cells, which are crucial for inhibiting scar formation.
