Generation of Trichogenic Adipose-Derived Stem Cells by Expression of Three Factors

August 2018 in “ Journal of Dermatological Science ”

Nahyun Choi, Jihun Choi, Ji Hye Kim, Y. S. Jang, Joo Hye Yeo, Juwon Kang, Seung Yong Song, Jinu Lee, Jong Hwan Sung

trichogenic adipose-derived stem cells adipose-derived stem cells PDGF-A SOX2 β-catenin dermal papilla cells hair follicle formation telogen phase anagen phase hair-regenerative potential hair regeneration therapy tfASCs ASCs platelet-derived growth factor A stem cell factor SOX2 beta-catenin DP cells hair growth phases hair growth therapy

Studysummary This study observed that adipose-derived stem cells modified with trichogenic factors have similar properties to dermal papilla cells and show enhanced hair regeneration potential compared to unmodified adipose-derived stem cells.

Our plain-language summary. Not medical advice or a treatment recommendation. Consult a qualified healthcare professional before changing treatment. Full disclaimer

The study from October 1, 2018, investigated the creation of trichogenic adipose-derived stem cells (tfASCs) by genetically modifying adipose-derived stem cells (ASCs) to overexpress three factors: PDGF-A, SOX2, and β-catenin. The tfASCs exhibited characteristics similar to dermal papilla (DP) cells, crucial for hair follicle formation, such as smaller size, shorter doubling time, resistance to aging, and the ability to be expanded until passage 12. They also showed increased cell cycle activity, higher mRNA levels of cell cycle-related genes, and a gene expression profile similar to DP cells. In vivo, tfASCs accelerated the transition from the telogen to anagen phase in hair follicles and induced new hair follicle formation in nude mice. The study concluded that tfASCs have enhanced hair-regenerative potential and could be promising for hair regeneration therapy. The number of mice used in one of the experiments was 6 per group.

View this study on jdsjournal.com →

Discuss this study in the Community →

Research cited in this study

25 / 25 results

Induction of Hair Follicle Dermal Papilla Cell Properties in Human Induced Pluripotent Stem Cell-Derived Multipotent LNGFR(+)THY-1(+) Mesenchymal Cells

research Induction of Hair Follicle Dermal Papilla Cell Properties in Human Induced Pluripotent Stem Cell-Derived Multipotent LNGFR(+)THY-1(+) Mesenchymal Cells

42 citations , February 2017 in “Scientific Reports”

In this study, researchers differentiated induced pluripotent stem cells into cells with dermal papilla-like properties, demonstrating their potential role in hair follicle bioengineering and drug testing for hair growth.

research Skin Adipocyte Stem Cell Self-Renewal Is Regulated by a PDGFA/AKT Signaling Axis

106 citations , October 2016 in “Cell Stem Cell”

This study found that PDGFA is critical for the proliferation and maintenance of adipocyte stem cells in skin but not other white adipose tissues, highlighting unique regulatory mechanisms in different tissue depots.

research LL-37 Stimulates the Functions of Adipose-Derived Stromal/Stem Cells via Early Growth Response 1 and the MAPK Pathway

53 citations , April 2016 in “Stem cell research & therapy”

This study reports that LL-37 treatment enhances cell proliferation, migration, and secretion of regenerative factors in adipose-derived stem cells, potentially promoting hair growth in vivo.

research SFRP2 Augments Wnt/β-Catenin Signalling in Cultured Dermal Papilla Cells

17 citations , February 2016 in “Experimental Dermatology”

This study found that SFRP2 enhances Wnt3a-mediated β-catenin signaling in human dermal papilla cells, with higher SFRP2 expression in beard cells correlating with increased trichogenicity.

research Poor Capability of 3D-Cultured Adipose-Derived Stem Cells to Induce Hair Follicles in Contrast to 3D-Cultured Dermal Papilla Cells

10 citations , January 2016 in “Annals of Dermatology”

This article discusses the factors contributing to acne and reports no new findings, focusing on the role of sebaceous lipids and associated processes.

research Clinical Use of Conditioned Media of Adipose Tissue-Derived Stem Cells in Female Pattern Hair Loss: A Retrospective Case Series Study

83 citations , March 2015 in “International Journal of Dermatology”

This retrospective study found that adipose-derived stem cell-conditioned media increased hair density and thickness in women with female pattern hair loss over 12 weeks, with no severe adverse effects reported.

research Functional Regulation of Adipose-Derived Stem Cells by PDGF-D

69 citations , October 2014 in “Stem Cells”

This study found that PDGF-D acts as a potent mitogenic factor in adipose-derived stem cells, enhancing their proliferation, migration, and growth factor expression, suggesting its use as a preconditioning agent for ASC transplantation.

$Isolation of Adipose-Derived Stem Cells Using a Subfractionation Culturing Method$

research Isolation of Adipose-Derived Stem Cells Using a Subfractionation Culturing Method

17 citations , July 2014 in “Expert Opinion on Biological Therapy”

This study concluded that the new subfractionation culturing method generates highly homogeneous adipose-derived stem cells with enhanced mitogenic, paracrine, and hair growth-promoting effects compared to traditional isolation methods.

research The Molecular Mechanism Underlying the Proliferating and Preconditioning Effect of Vitamin C on Adipose-Derived Stem Cells

42 citations , February 2014 in “Stem Cells and Development”

This study found that vitamin C increased the survival, proliferation, and hair-regenerative potential of adipose-derived stem cells through the MAPK pathway and enhanced hair growth in mice.

Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

research Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

256 citations , October 2013 in “Proceedings of the National Academy of Sciences of the United States of America”

This study found that altering cell culture conditions to form three-dimensional papilla spheroids can restore the ability of human dermal papilla cells to induce hair growth in adult skin.

research Valproic Acid Promotes Human Hair Growth in In Vitro Culture Model

48 citations , June 2013 in “Journal of Dermatological Science”

This study found that valproic acid promoted human hair growth by increasing β-catenin levels, suggesting it may be a potential therapeutic option for alopecia.

research Human Induced Pluripotent Stem Cell-Derived Ectodermal Precursor Cells Contribute to Hair Follicle Morphogenesis In Vivo

64 citations , January 2013 in “The journal of investigative dermatology/Journal of investigative dermatology”

This study found that ectodermal precursor cells derived from human induced pluripotent stem cells may enhance hair follicle morphogenesis through improved epithelial-mesenchymal interactions when cocultured with dermal cells.

Ultraviolet B Preconditioning Enhances the Hair Growth-Promoting Effects of Adipose-Derived Stem Cells Via Generation of Reactive Oxygen Species

research Ultraviolet B Preconditioning Enhances the Hair Growth-Promoting Effects of Adipose-Derived Stem Cells Via Generation of Reactive Oxygen Species

41 citations , July 2012 in “Stem Cells and Development”

This study found that low-dose UVB radiation enhances the survival, migration, and hair growth-promoting capacity of adipose-derived stem cells in mice through the generation of reactive oxygen species.

research Adipocyte Lineage Cells Contribute to the Skin Stem Cell Niche to Drive Hair Cycling

499 citations , September 2011 in “Cell”

This study found that intradermal adipocyte lineage cells in mice are necessary and sufficient to activate follicular stem cells, highlighting their role in regulating skin stem cell activity.

Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay

research Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay

75 citations , August 2011 in “Journal of Investigative Dermatology”

This study found that cultured human dermal papilla cells formed into three-dimensional spheres have an increased ability to induce hair follicles from mouse epidermal cells compared to two-dimensional cultures.

research Minoxidil Activates β-Catenin Pathway in Human Dermal Papilla Cells: A Possible Explanation for Its Anagen Prolongation Effect

88 citations , February 2011 in “Journal of Dermatological Science”

This study found that topical minoxidil modestly extended the anagen phase in mice by activating β-catenin activity in dermal papilla cells.

research Beta-Catenin Activity in the Dermal Papilla Regulates Morphogenesis and Regeneration of Hair

314 citations , April 2010 in “Developmental Cell”

This study found that in mice, inactivating beta-catenin in the dermal papilla reduces hair follicle progenitor proliferation and disrupts the hair cycle.

research Hair Growth Promoting Effects of Adipose Tissue-Derived Stem Cells

81 citations , December 2009 in “Journal of Dermatological Science”

This review discusses the paracrine effects of adipose tissue-derived stem cells on surrounding cells and tissues, noting their potential therapeutic benefits, but reports no new clinical results.

research SKPs Derive from Hair Follicle Precursors and Exhibit Properties of Adult Dermal Stem Cells

330 citations , December 2009 in “Cell stem cell”

This study suggests that skin-derived precursors from Sox2(+) hair follicle dermal cells have the potential to function as dermal stem cells, supporting hair morphogenesis, dermal maintenance, and wound-healing.

research Sox2-Positive Dermal Papilla Cells Specify Hair Follicle Type in Mammalian Epidermis

338 citations , July 2009 in “Development”

This study demonstrates that Sox2-positive dermal papilla cells specify particular hair follicle types by showing heterogeneity in gene expression and pathway activation in these cells.

research Keratinocyte Growth Inhibition Through the Modification of Wnt Signaling by Androgen in Balding Dermal Papilla Cells

70 citations , January 2009 in “The Journal of clinical endocrinology and metabolism/Journal of clinical endocrinology & metabolism”

This study explored the interaction between androgen and Wnt signaling in dermal papilla cells, targeting the effects of androgen on hair growth reduction.

Self-Assembly of Dermal Papilla Cells into Inductive Spheroidal Microtissues on Poly(Ethylene-Co-Vinyl Alcohol) Membranes for Hair Follicle Regeneration

research Self-Assembly of Dermal Papilla Cells into Inductive Spheroidal Microtissues on Poly(Ethylene-Co-Vinyl Alcohol) Membranes for Hair Follicle Regeneration

80 citations , June 2008 in “Biomaterials”

This study found that poly(ethylene-co-vinyl alcohol) membranes support the self-assembly of dermal papilla cells into compact spheroidal microtissues capable of inducing new hair follicles, suggesting potential for large-scale production for hair follicle regeneration.

research PDGF Isoforms Induce and Maintain Anagen Phase of Murine Hair Follicles

136 citations , June 2006 in “Journal of Dermatological Science”

This study found that injecting PDGF-AA and -BB into mouse skin immediately triggered the hair growth cycle's anagen phase, suggesting potential treatment options for certain types of alopecia.

research Organogenesis From Dissociated Cells: Generation of Mature Cycling Hair Follicles From Skin-Derived Cells

205 citations , April 2005 in “Journal of Investigative Dermatology”

This study describes a system for generating hair follicles from dissociated cells in mice, suggesting that successful development requires a foundational epithelial platform.

research Growth Factor and Growth Factor Receptor Localization in the Hair Follicle Bulge and Associated Tissue in Human Fetus

94 citations , March 1996 in “Journal of Investigative Dermatology”