Self-Assembled Complete Hair Follicle Organoids by Coculture of Neonatal Mouse Epidermal Cells and Dermal Cells in Matrigel

The study used neonatal mouse epidermal and dermal cells to create hair follicle organoids in a 3D organoid culture. These organoids closely resembled the structure and molecular markers of native hair follicles, including the outer root sheath, inner root sheath, hair shaft, and dermal papillae. They also expressed hair follicle-related markers and stem cell-related markers. The organoids were only self-assembled in Matrigel, a common biomaterial scaffold, and further research is needed to determine if other 3D structure generation methods would yield similar results. This research could potentially contribute to the development of treatments for hair loss and provide new insights into hair health and regeneration.