Glycome Profiling by Lectin Microarray Reveals Dynamic Glycan Alterations During Epidermal Stem Cell Aging

July 2020 in “ Aging Cell ”

Lalhaba Oinam, Gopakumar Changarathil, Erna Raja, Yen Xuan Ngo, Hiroaki Tateno, Aiko Sada, Hiromi Yanagisawa

TLDR Aging changes sugar molecules on skin stem cells, which may affect their ability to repair skin.

The study investigated glycan alterations in epidermal stem cells during aging using lectin microarray profiling in young (2 months) and old (22-24 months) mice. It found that aging is associated with a shift from high mannose-type N-glycans to α2-3/6 sialylated complex-type N-glycans. Gene expression analysis revealed upregulation of sialyltransferase (St3gal2, St6gal1) and mannosidase (Man1a) genes in old stem cells. Overexpression of these genes in primary keratinocytes mimicked aging glycan patterns and impaired cell proliferation, suggesting that glycan changes contribute to the decline in stem cell function with age. The study proposed using specific lectins as probes for aged stem cells, with potential applications in regenerative therapy and skin aging diagnosis.

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