Rat Hair Follicle Stem Cell-Derived Exosomes: Isolation, Characterization, and Comparative Analysis of Their In Vitro Wound Healing Potential

May 2025 in “ International Journal of Molecular Sciences ”

Patrícia Sousa, Bruna Lopes, Ana Catarina Sousa, Alícia Moreira, Alexandra Rêma, Rui Alvites, Stefano Geuna, Nuno Alves, Ana Colette Maurício

exosomes hair follicle stem cells wound healing IL-6 VEGF MCP-1 angiogenesis tissue regeneration regenerative medicine cell migration cell proliferation wound closure interleukin-6 vascular endothelial growth factor monocyte chemoattractant protein-1

TLDR Exosomes from rat hair follicle stem cells may help heal wounds and regenerate skin.

This study investigates the wound healing potential of exosomes derived from rat hair follicle stem cells (rHFSCs). The exosomes were isolated and characterized, revealing a unique biomolecule composition that promotes cell migration, proliferation, and wound closure in vitro. Key bioactive molecules like IL-6, VEGF, and MCP-1 were identified, which are crucial for inflammation, angiogenesis, and tissue regeneration. The study highlights the therapeutic promise of rHFSC-derived exosomes in regenerative medicine, suggesting their potential as innovative, cell-free therapeutic agents for skin regeneration and wound healing. Further research is needed to understand the molecular pathways involved and to optimize exosome-based therapies.

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