Force-Triggered Density Gradient Sedimentation and Cocktail Enzyme Digestion Treatment for Isolation of Single Dermal Papilla Cells from Follicular Unit Extraction Harvesting Human Hair Follicles

    October 2024
    Junfei Huang, Jian Chen, Haoyuan Li … Lijuan Du
    Studysummary This study found that new methods for isolating human dermal papilla cells were more efficient than traditional methods, maintaining higher levels of specific markers and improving the cells' research potential.
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    Research cited in this study 20

    1. Regenerative Medicine in the Treatment of Specific Dermatologic Disorders: A Systematic Review of Randomized Controlled Clinical Trials Stem cell research & therapy · 2024
    2. Status of Research on the Development and Regeneration of Hair Follicles International Journal of Medical Sciences · 2023
    3. An Optimized Force-Triggered Density Gradient Sedimentation Method for Isolation of Pelage Follicle Dermal Papilla Cells from Neonatal Mouse Skin Stem cell research & therapy · 2023
    4. Mechanical Force Drives the Initial Mesenchymal-Epithelial Interaction During Skin Organoid Development Theranostics · 2023
    5. Regenerative Medicine Strategies for Hair Growth and Regeneration: A Narrative Review of Literature Regenerative Therapy · 2022
    6. Ficoll Density Gradient Sedimentation Isolation of Pelage Hair Follicle Mesenchymal Stem Cells From Adult Mouse Back Skin: A Novel Method for Hair Follicle Mesenchymal Stem Cells Isolation Stem cell research & therapy · 2022
    7. Generation And Characterization Of Hair-Bearing Skin Organoids From Human Pluripotent Stem Cells Nature Protocols · 2022
    8. Cellular Nanofiber Structure with Secretory Activity-Promoting Characteristics for Multicellular Spheroid Formation and Hair Follicle Regeneration ACS Applied Materials & Interfaces · 2020
    9. Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt Pathway, Growth Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development Cells · 2019
    10. Review of Hair Follicle Dermal Papilla Cells as In Vitro Screening Model for Hair Growth International Journal of Cosmetic Science · 2018
    11. Subpopulations of Dermal Skin Fibroblasts Secrete Distinct Extracellular Matrix: Implications for Using Skin Substitutes in the Clinic British Journal of Dermatology · 2017
    12. Methods for the Isolation and 3D Culture of Dermal Papilla Cells from Human Hair Follicles Experimental Dermatology · 2017
    13. Hair Follicle Dermal Stem Cells Regenerate the Dermal Sheath, Repopulate the Dermal Papilla, and Modulate Hair Type Developmental Cell · 2014
    14. Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth Proceedings of the National Academy of Sciences of the United States of America · 2013
    15. Live Imaging of Stem Cell and Progeny Behavior in Physiological Hair-Follicle Regeneration Nature · 2012
    16. SKPs Derive from Hair Follicle Precursors and Exhibit Properties of Adult Dermal Stem Cells Cell stem cell · 2009
    17. Cultured Dermal Papilla Cells of the Rat Vibrissa Follicle: Proliferative Activity, Adhesion Properties, and Reorganization of the Extracellular Matrix In Vitro Archives of Dermatological Research · 1997
    18. Hair Matrix Germinative Epidermal Cells Confer Follicle-Inducing Capabilities on Dermal Sheath and High Passage Papilla Cells Development · 1996
    19. Distribution of Extracellular Matrix Molecules in Human Hair Follicles Annals of the New York Academy of Sciences · 1991
    20. Rat Hair Follicle Dermal Papillae Have an Extracellular Matrix Containing Basement Membrane Components Journal of Investigative Dermatology · 1986

    Related research 7

    1. Possible Cellular Communication with Human Follicle Dermal Papilla Cells via Secretions from Human Hair Follicle Keratinocytes Journal of St. Marianna University · 2022
    2. A Model System to Analyze the Ability of Human Keratinocytes to Form Hair Follicles Experimental Dermatology · 2014
    3. Review of Hair Follicle Dermal Cells Journal of Dermatological Science · 2009
    4. Hair Follicle Reformation Induced by Dermal Papilla Cells from Human Scalp Skin Archives of Dermatological Research · 2006
    5. Study on Hair Follicle Formation Induced by Dermal Papilla Cells Chinese Journal of Reparative and Reconstructive Surgery · 2003
    6. Trans-Species Hair Growth Induction by Human Hair Follicle Dermal Papillae Experimental Dermatology · 2001
    7. Role of Hair Papilla Cells in Induction and Regeneration Processes of Hair Follicles Wound Repair and Regeneration · 1998