An Optimized Force-Triggered Density Gradient Sedimentation Method for Isolation of Pelage Follicle Dermal Papilla Cells from Neonatal Mouse Skin

May 2023 in “ Stem cell research & therapy ”

Lijuan Du, Yuyang Gan, Bowen Zheng, Junfei Huang, Zhiqi Hu, Yong Miao

force-triggered density gradient sedimentation dermal papilla cells neonatal mouse skin alkaline phosphatase β-catenin versican NCAM hair regenerative capacity hair follicle biology regenerative medicine FDGS DP cells hair regeneration

TLDR New method efficiently isolates hair growth cells from newborn mouse skin.

The study introduces an optimized force-triggered density gradient sedimentation (FDGS) method for isolating dermal papilla (DP) cells from neonatal mouse skin, demonstrating improved efficiency and purity over traditional methods. The isolated DP cells expressed key markers (alkaline phosphatase, β-catenin, versican, NCAM) and retained their hair regenerative capacity in vivo. This method simplifies the isolation process, making it more efficient and scalable, thus enhancing the potential for research in hair follicle biology and regenerative medicine. The study was validated through various experiments and approved by the Animal Ethics Committee of Nanfang Hospital of Southern Medical University.

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research Ficoll Density Gradient Sedimentation Isolation of Pelage Hair Follicle Mesenchymal Stem Cells From Adult Mouse Back Skin: A Novel Method for Hair Follicle Mesenchymal Stem Cells Isolation

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Understanding how hair follicle stem cells work can help find new ways to prevent hair loss and promote hair growth.

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The review concluded that keeping the hair-growing ability of human dermal papilla cells is key for hair development and growth.

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The method successfully isolates cells that are important for hair growth and could help study hair loss.

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Dermal Papilla cells are a promising tool for evaluating hair growth treatments.

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The document concludes that understanding dermal papilla cells is key to improving hair regeneration treatments.

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Dermal cells are key in controlling hair growth and could potentially be used in hair loss treatments, but more research is needed to improve hair regeneration methods.

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Adult mouse skin contains stem cells that can create new hair, skin, and oil glands.