Hair Growth Is Promoted in Mouse Dorsal Skin by a Mixture of Platycladus Orientalis Leaf Extract and Alpha-Terpineol by Increasing Growth Factors and Wnt3/β-Catenin

In a study involving 14 C57/BL6 mice, a mixture of Platycladus orientalis leaf extract and alpha-terpineol (PEaT) was applied topically to the dorsal skin for 17 days, resulting in significant promotion of hair growth. The PEaT-treated mice showed an early transition to the anagen phase of hair growth, with about 70% hair coverage by day 17. The treatment also led to a substantial increase in cell proliferation, as indicated by a 298% rise in Ki-67-positive cells, and enhanced expression of growth factors, with IGF-1 and VEGF increasing by approximately 329% and 200%, respectively. Additionally, the study found increased expressions of wnt3 and β-catenin, which are critical for hair follicle development, by about 22% and 109%, respectively. These results suggest that PEaT could be an effective agent for hair growth by boosting growth factors and cell proliferation. The study was supported by Leefarm Co., Ltd., with each group containing 7 mice.