A Fast and Efficient UPLC-ESI-MS/MS Method for Detection, Identification, and Quantitative Analysis of Bioactive Substances in Medicinal Herbal Extracts for Preventing Hair Loss
September 2015
in “
Bulletin of the Korean Chemical Society
”
Studysummary This study presents a new method using UPLC-ESI-MS/MS for analyzing the constituents of a medicinal herbal complex extract, which includes herbs traditionally used to prevent hair loss and promote hair growth; no clinical results were reported.
Our plain-language summary. Not medical advice or a treatment recommendation. Consult a qualified healthcare professional before changing treatment. Full disclaimer
In 2015, researchers developed a rapid and sensitive UPLC-ESI-MS/MS method for both qualitative and quantitative analysis of bioactive substances in a medicinal herbal complex extract (MHCE), which was composed of 23 herbs traditionally used in oriental medicine to prevent hair loss and promote hair growth. The method utilized a reversed-phase C18 column and gradient elution for chromatographic analysis, with mass spectrometry conducted in the positive ESI mode. The detection of bioactive compounds was achieved through selected reaction monitoring (SRM). The method demonstrated high linearity (r2 > 0.9964) for the calibration curves of reference compounds, and the limits of detection and quantification were notably low at 0.02 ng/mL and 0.05 ng/mL, respectively.
