Canine Adipose-Derived Stem Cell Aggregates as a Viable Substitute to Actual Canine Dermal Papillae

May 2015 in “ Macedonian Veterinary Review ”

Sohee Bae, Jina Kim, Li Li, Aeri Lee, Hyunjoo Lim, Junemoe Jeong, Seung‐Hoon Lee, Oh‐Kyeong Kweon, Wan Hee Kim

adipose-derived mesenchymal stem cells dermal papilla-like tissue dermal papillae hair regeneration dermal papilla cells gene expression protein expression RT-PCR analysis Western blotting hair follicle biology alopecia stem cells DPLT DP DPCs hair loss

TLDR Canine stem cell aggregates can effectively replace natural dermal papillae for hair research.

This study explored the potential of using canine adipose-derived mesenchymal stem cells (AD-MSCs) to create dermal papilla-like tissue (DPLT) as an alternative to actual canine dermal papillae (DP) for hair regeneration research. The researchers successfully reconstructed DPLTs, which were morphologically and molecularly similar to natural DPs. The study involved culturing canine dermal papilla cells (DPCs) and comparing their gene and protein expression to that of DPLTs using RT-PCR analysis and Western blotting. The findings indicated that DPLTs could serve as a viable substitute for DPs, enhancing the understanding of canine hair follicle biology and potentially informing therapeutic strategies for human alopecia.

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