Investigation of Transcriptional Gene Profiling in Normal Murine Hair Follicular Substructures Using Next-Generation Sequencing to Provide Potential Insights into Skin Disease
February 2016
in “
Cell Transplantation
”
next-generation sequencing RNA-seq interfollicular keratinocytes hair follicles dermal fibroblasts dermal papilla cells C57BL/6 murine skin differentially expressed genes gene ontology quiescent cell cycle signaling pathways hair follicle-related diseases genetically targeted therapies bioengineering applications NGS RNA sequencing IFKs HFs DFs DPCs mouse skin DEGs gene function cell cycle signaling hair diseases targeted therapies bioengineering
TLDR Hair follicles have a more inactive cell cycle than other skin cells, which may help develop targeted therapies for skin diseases and cancer.
The study used next-generation sequencing to analyze gene expression in interfollicular keratinocytes (IFKs), hair follicles (HFs), and dermal fibroblast-dermal papilla cells (DF-DPCs) from normal C57BL/6 murine skin. Transplantation of these cells into nude mice showed sustained hair growth supported by HFs and DF-DPCs. The research identified differentially expressed genes (DEGs) and analyzed pathways and gene ontology, finding that HFs had a more quiescent cell cycle pattern compared to IFKs and DF-DPCs. Key genes related to hair cycle and follicle development were upregulated in HFs. The study provided insights into gene profiling of skin keratinocytes and potential interactions in HF-related diseases and cancer, aiding future bioengineering applications.
