Investigation of Transcriptional Gene Profiling in Normal Murine Hair Follicular Substructures Using Next-Generation Sequencing to Provide Potential Insights into Skin Disease

February 2016 in “ Cell Transplantation ”

Jae-In Lee, Kang-In Lee, Hyung‐Min Chung

Studysummary In this study, researchers found that hair follicles and dermal fibroblasts, including dermal papilla cells, supported sustained hair growth in transplanted murine models, with RNA-seq analysis revealing active signaling pathways and gene expression patterns. Our plain-language summary of this paper — not a Tressless recommendation.

The study used next-generation sequencing to analyze gene expression in interfollicular keratinocytes (IFKs), hair follicles (HFs), and dermal fibroblast-dermal papilla cells (DF-DPCs) from normal C57BL/6 murine skin. Transplantation of these cells into nude mice showed sustained hair growth supported by HFs and DF-DPCs. The research identified differentially expressed genes (DEGs) and analyzed pathways and gene ontology, finding that HFs had a more quiescent cell cycle pattern compared to IFKs and DF-DPCs. Key genes related to hair cycle and follicle development were upregulated in HFs. The study provided insights into gene profiling of skin keratinocytes and potential interactions in HF-related diseases and cancer, aiding future bioengineering applications.

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Treatment and Prevention of Chemotherapy-Induced Alopecia with PTH-CBD, a Collagen-Targeted Parathyroid Hormone Analog, in a Non-Depilated Mouse Model

research Treatment and Prevention of Chemotherapy-Induced Alopecia with PTH-CBD, a Collagen-Targeted Parathyroid Hormone Analog, in a Non-Depilated Mouse Model

20 citations , September 2013 in “Anti-Cancer Drugs”

In this study, PTH-CBD effectively prevented and partially reversed chemotherapy-induced alopecia in mice, with pretreatment offering a better cosmetic outcome compared to therapeutic administration after hair loss onset.

research Serum Granulysin as a Possible Key Marker of the Activity of Alopecia Areata

26 citations , September 2013 in “Journal of Dermatological Science”

In this study, researchers found that serum granulysin levels might serve as a novel marker for disease activity in acute alopecia areata, being significantly associated with broader bald skin areas, poorer prognosis, and co-existing allergic disorders.

research Tuning Wnt Signals for More or Fewer Hairs

39 citations , January 2013 in “Journal of Investigative Dermatology”

This review explains how recent understanding of Wnt signaling regulation in hair follicles could potentially be used to enhance hair growth, but it reports no new empirical results.

research Generation and Characterization of Multipotent Stem Cells from Established Dermal Cultures

58 citations , November 2012 in “PLoS ONE”

This study found that dermal fibroblast cultures, especially those with higher alpha-smooth muscle actin expression, can be used to produce skin-derived precursor cells, unlike human hair follicle dermal papilla cultures.

research Canine Noninflammatory Alopecia: A Comprehensive Evaluation of Common and Distinguishing Histological Characteristics

28 citations , May 2012 in “Veterinary Dermatology”

In this study, dogs with hair cycle disorders, especially those with alopecia X, showed a significant increase in the number of kenogen follicles, suggesting impaired induction of new hair growth phases.

research Regulation of Hair Shedding by the Type 3 IP3 Receptor

19 citations , May 2012 in “The journal of investigative dermatology/Journal of investigative dermatology”

This study found that mice lacking the type 3 IP(3) receptor in their hair follicles experienced repetitive hair loss and regrowth, indicating disrupted hair-cycle regulation potentially linked to specific signaling pathways.

research Sox2-Positive Dermal Papilla Cells Specify Hair Follicle Type in Mammalian Epidermis

338 citations , July 2009 in “Development”

This study demonstrates that Sox2-positive dermal papilla cells specify particular hair follicle types by showing heterogeneity in gene expression and pathway activation in these cells.

research Isolation and Short-Term Culture of Primary Keratinocytes, Hair Follicle Populations, and Dermal Cells from Newborn Mice and Keratinocytes from Adult Mice for In Vitro Analysis and Grafting to Immunodeficient Mice

427 citations , April 2008 in “Nature Protocols”

research Maintenance of Hair Follicle Immune Privilege Is Linked to Prevention of NK Cell Attack

253 citations , December 2007 in “Journal of Investigative Dermatology”

This study suggests that human hair follicles may actively suppress natural killer cells, which contrasts with the observed defects in NK cell regulation in alopecia areata.

research Specific Citrullination Causes Assembly of a Globular S100A3 Homotetramer

62 citations , December 2007 in “Journal of biological chemistry/The Journal of biological chemistry”

This study found that enzymatic conversion of Arg-51 in S100A3 protein to citrulline promotes homotetramer assembly, potentially increasing Ca²⁺ binding required for hair cuticular barrier formation.

research Bulge Cells of Human Hair Follicles: Segregation, Cultivation, and Properties

27 citations , January 2006 in “Colloids and Surfaces B: Biointerfaces”

This study found that in vitro, cultured bulge cells from human hair follicles possess properties of primitive cells, supporting the hypothesis that hair follicle stem cells reside in the bulge area.

research Characterization and Isolation of Stem Cell-Enriched Human Hair Follicle Bulge Cells

550 citations , December 2005 in “The Journal of clinical investigation/The journal of clinical investigation”

This study found that bulge cells marked by CD200+ in human hair follicles show high colony-forming efficiency, suggesting successful enrichment of keratinocyte stem cells.

research Role of Androgen in Mesenchymal Epithelial Interactions in Human Hair Follicle

67 citations , August 2005 in “Journal of Investigative Dermatology Symposium Proceedings”

This study found that in vitro, beard dermal papilla cells stimulated keratinocyte proliferation via androgen-dependent growth factors, while androgenetic alopecia cells released TGF-β1, inhibiting keratinocyte growth.

research Molecular Mechanisms Regulating Hair Follicle Development

854 citations , February 2002 in “The journal of investigative dermatology/Journal of investigative dermatology”

This review summarizes recent advances in understanding the molecular mechanisms of hair follicle formation and discusses potential future clinical applications for treating hair loss and skin tumors, but it reports no new clinical results.

research Gene Expression of Mouse S100A3, a Cysteine-Rich Calcium-Binding Protein, in Developing Hair Follicle

40 citations , November 1998 in “The journal of investigative dermatology/Journal of investigative dermatology”

This study found that the S100A3 gene is exclusively expressed in hair follicle cells differentiating into hair shaft components in mice, suggesting its important role in hair formation.

Chemotherapy-Induced Alopecia in Mice: Induction by Cyclophosphamide, Inhibition by Cyclosporine A, and Modulation by Dexamethasone

research Chemotherapy-Induced Alopecia in Mice: Induction by Cyclophosphamide, Inhibition by Cyclosporine A, and Modulation by Dexamethasone

36 citations , April 1994 in “PubMed”

This study introduces a mouse model of cyclophosphamide-induced alopecia, which effectively replicates human chemotherapy-induced hair loss and can be pharmacologically manipulated to study hair regrowth patterns.