Gellan Gum-Based Hydrogels Support the Recreation of the Dermal Papilla Microenvironment

The study suggests that a 3D culture system using the RAD16-I peptide scaffold can help restore the original phenotype of hair follicle dermal papilla cells and support their osteogenic and adipogenic differentiation.

This study developed a method for generating germ-like tissues called bbHFGs, which efficiently promoted hair follicle formation in mice, potentially advancing hair regenerative medicine applications.

This study found that alginate spheres maintain dermal papilla cells in a dormant state with increased trichogenecity, suggesting potential benefits for cell therapy in androgenic alopecia.

This study found that using 3D dermal papilla spheroid models enhances extracellular matrix production and hair follicle marker expression, providing insights into hair follicle biology and potential for drug screening.

This study observed that dermal papilla cell aggregates showed increased viability and gene expression on softer 3D substrates, indicating the importance of substrate stiffness in cellular behavior.

This study reports that using nanogel and layer-by-layer self-assembly to encapsulate single dermal papilla cells can form cell spheroids that regenerate hair follicles successfully in a hair follicle regeneration model.

This article details laboratory methods for isolating and culturing human dermal papilla cells but provides no new experimental results.

This study found that 3D microtissue models of dermal papilla cells can enhance the ability to induce hair-follicle neogenesis in vivo, offering potential for controlled cell production in follicle regeneration.

This study found that a 3D Matrigel culture technique for dermal papilla cell spheroids can enhance hair follicle inductivity and induce hair-like fiber differentiation in vitro, even with high-passage cells.

This study found that altering cell culture conditions to form three-dimensional papilla spheroids can restore the ability of human dermal papilla cells to induce hair growth in adult skin.

This study demonstrated that cultured human dermal papilla cells can induce full hair follicle formation when combined with neonatal foreskin keratinocytes in a grafted dermal-epidermal construct on mice.

This study developed a 3D engineered hair follicle model that mimics the native hair bulb and may aid in drug screening for hair growth therapies.

This study found that both human and rat dermal papilla spheroids can induce hair follicle neogenesis, but larger spheroids increase inductivity without significantly affecting hair fiber diameter.

This review discusses mesenchymal–epithelial interactions in hair follicle development and cycling, highlighting recent insights without reporting new experimental results.

This study reported that a three-dimensional hydrogel culture system supports the growth and maintenance of distinct dermal papilla cell types, crucial for skin reconstitution assays in neonatal mice.

Skin cells can help create early hair-like structures in lab cultures.

This review discusses the role of dermal cells in hair follicle regeneration and morphogenesis and highlights their potential therapeutic implications, but it reports no new empirical findings.

This study presents a novel in vitro assay using human folliculoid microspheres to research hair growth, which may facilitate preclinical testing of hair growth-modulatory agents.

This study found that poly(ethylene-co-vinyl alcohol) membranes support the self-assembly of dermal papilla cells into compact spheroidal microtissues capable of inducing new hair follicles, suggesting potential for large-scale production for hair follicle regeneration.

This study found that transplanted microencapsulated dermal papilla cells in rat footpads can regenerate hair follicles and sebaceous gland structures, suggesting a potential substitute for fresh dermal papillae cells.

This study found that dermal papilla and sheath cells can induce hair follicle formation in vitro and in vivo by interacting with hair follicle epithelial cells.

In this study, specific versican expression was observed in the anagen phase of human hair follicles, suggesting its importance in maintaining normal hair growth.

This review introduces the morphological and molecular principles of hair follicle development but reports no new experimental results, focusing on recent insights and forming a working hypothesis.

This article reviews the role of epithelial-mesenchymal interactions in hair follicle morphogenesis and the hair cycle, suggesting potential paths for developing treatments for hair growth disorders but providing no new clinical findings.

This review summarizes recent advances in understanding the molecular mechanisms of hair follicle formation and discusses potential future clinical applications for treating hair loss and skin tumors, but it reports no new clinical results.

This study found that the volume of a hair follicle's dermal papilla is strongly correlated with its size, primarily due to the number of cells it contains and the volume of extracellular matrix.

In this study, researchers found that changes in the extracellular matrix components, particularly fibronectin, laminin, and type IV collagen, during the rat vibrissa follicle growth cycle, suggest dynamic remodeling related to dermal-epidermal signaling.

This article describes the potential role of various growth factors and molecules in regulating mammalian hair follicle development but does not present new experimental results.

This article discusses the distribution of extracellular matrix molecules in human hair follicles and reports no new results.

Epithelial cell growth in rat hair follicles needs contact with mesenchymal cells through the extracellular matrix.

Fibronectin and other basement membrane components increase during hair growth and decrease during rest.