Bulge-Derived Epithelial Cells Isolated From Human Hair Follicles Using Enzymatic Digestion Or Explants Result In Comparable Tissue-Engineered Skin

    February 2025 in “ International Journal of Molecular Sciences ”
    Bettina Cattier, Rina Guignard, I. Martel … Lucie Germain
    Studysummary This study found that an enzymatic digestion method for isolating hair follicle stem cells from scalp specimens yielded 5.3 times more epithelial cells than explant cultures, while supporting comparable cell functionality, offering a promising technique for tissue engineering and regenerative medicine.
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    Research cited in this study 26

    1. Hair Regeneration Methods Using Cells Derived From Human Hair Follicles and Challenges to Overcome Cells · 2024
    2. Generating Induced Pluripotent Stem Cells with a High-Efficiency, Non-Invasive Method: An Improved Way to Cultivate Keratinocytes from Plucked Hair for Reprogramming Cells · 2022
    3. Functional Hair Follicle Regeneration: An Updated Review Signal Transduction and Targeted Therapy · 2021
    4. Hair Follicle Bulge-Derived Stem Cells Promote Tissue Regeneration During Skin Expansion Biomedicine & Pharmacotherapy · 2020
    5. Isolation, Cultivation, and Morphological Characteristics of Hair Follicle Adult Stem Cells in the Bulge Region in Mouse and Human Microscopy research · 2020
    6. Tissue Engineering of Human Hair Follicles Using a Biomimetic Developmental Approach Nature Communications · 2018
    7. Concise Review: Wnt Signaling Pathways in Skin Development and Epidermal Stem Cells Stem cells · 2017
    8. Induction of Osteoblastic Differentiation of Neural Crest-Derived Stem Cells from Hair Follicles PLoS ONE · 2017
    9. From Hair to Heart: Nestin-Expressing Hair-Follicle-Associated Pluripotent Stem Cells Differentiate to Beating Cardiac Muscle Cells Cell Cycle · 2015
    10. Markers of Epidermal Stem Cell Subpopulations in Adult Mammalian Skin Cold Spring Harbor Perspectives in Medicine · 2014
    11. Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth Proceedings of the National Academy of Sciences of the United States of America · 2013
    12. Dissecting the Bulge in Hair Regeneration Journal of Clinical Investigation · 2012
    13. Towards Expansion of Human Hair Follicle Stem Cells In Vitro Cell Proliferation · 2011
    14. Considerations in the Choice of a Skin Donor Site for Harvesting Keratinocytes Containing a High Proportion of Stem Cells for Culture In Vitro Burns · 2010
    15. Tissue-Engineered Skin Preserving the Potential of Epithelial Cells to Differentiate into Hair After Grafting Tissue Engineering Part A · 2010
    16. Differential Expression of Stem Cell Markers in Human Follicular Bulge and Interfollicular Epidermal Compartments Histochemistry and Cell Biology · 2010
    17. The Hair Follicle as a Dynamic Mini-Organ Current Biology · 2009
    18. Neural Potential of a Stem Cell Population in the Hair Follicle Cell Cycle · 2007
    19. Characterization and Isolation of Stem Cell-Enriched Human Hair Follicle Bulge Cells ˜The œJournal of clinical investigation/˜The œjournal of clinical investigation · 2005
    20. Implanted Hair Follicle Stem Cells Form Schwann Cells That Support Repair of Severed Peripheral Nerves Proceedings of the National Academy of Sciences · 2005
    21. Multipotent Nestin-Positive, Keratin-Negative Hair-Follicle Bulge Stem Cells Can Form Neurons Proceedings of the National Academy of Sciences · 2005
    22. Human Follicular Stem Cells: Their Presence in Plucked Hair and Follicular Cell Culture British Journal of Dermatology · 2004
    23. Keratin 15 Promoter Targets Putative Epithelial Stem Cells in the Hair Follicle Bulge Journal of Investigative Dermatology · 2003
    24. Epithelial Barrier Function: Assembly and Structural Features of the Cornified Cell Envelope BioEssays · 2002
    25. Characterization of a New Tissue-Engineered Human Skin Equivalent with Hair In Vitro Cellular & Developmental Biology - Animal · 1999
    26. Keratin 19 as a Biochemical Marker of Skin Stem Cells In Vivo and In Vitro: Localization and Variation with Age and Culture Stage Journal of Cell Science · 1996