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    Glossary Ex Vivo Culture

    growing cells or tissues outside their original biological context

    Ex Vivo Culture refers to the process of growing or maintaining cells, tissues, or organs outside of their original biological context, typically in a controlled laboratory environment. This technique allows researchers to study biological processes in a more natural state than in vitro (in a petri dish) while still being able to manipulate and observe the sample closely. For example, hair follicles can be cultured ex vivo to study hair growth and alopecia treatments.

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    1. A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans Scientific reports · 2017 · 12 citations
    2. Human hair growth ex vivo is correlated with in vivo hair growth: selective categorization of hair follicles for more reliable hair follicle organ culture Archives of Dermatological Research · 2005 · 27 citations
    3. Ex Vivo Organ Culture of Human Hair Follicles: A Model Epithelial–Neuroectodermal–Mesenchymal Interaction System Methods in molecular biology · 2010 · 13 citations
    4. Prostaglandin F2α stimulates the growth of human intermediate hair follicles in ex vivo organ culture with potential clinical relevance Frontiers in Physiology · 2025
    5. Identification of human hair follicle antigens targeted in the presumptive autoimmune hair follicle disorder alopecia areata and their potential functional relevance in vitro : methods development for isolation and identification of alopecia areata-relevant human hair follicle antigens using a proteomics approach and their functional assessment using an ex vivo hair follicle organ culture model Bradford Scholars (University of Bradford) · 2008
    6. 3D cell culture stimulates the secretion of in vivo like extracellular vesicles bioRxiv (Cold Spring Harbor Laboratory) · 2019 · 5 citations
    7. Ex-vivo Hair Growth Promotion Efficacy of Biofield Energy Treated Williams Medium E using Vibrissae Hair Follicle Organ Culture Zenodo (CERN European Organization for Nuclear Research) · 2018
    8. Keratin 19 as a biochemical marker of skin stem cells in vivo and in vitro: keratin 19 expressing cells are differentially localized in function of anatomic sites, and their number varies with donor age and culture stage Journal of Cell Science · 1996 · 441 citations
    9. 581 Spontaneous, Intrinsic Aging in Organ-Cultured Human Skin as Novel Ex Vivo Assay to Test Candidate Anti-Aging Actives Journal of Investigative Dermatology · 2022
    10. Biological characterization of cultured dermal papilla cells and hair follicle regeneration in vitro and in vivo Chinese Medical Journal · 2006 · 24 citations
    11. Ability to culture dermal papilla cells from red deer (Cervus elaphus) hair follicles with differing hormonal responses in vivo offers a new model for studying the control of hair follicle biology 1996 · 16 citations
    12. Cultured nail keratinocytes express hard keratins characteristic of nail and hair in vivo Archives of dermatological research · 1992 · 16 citations
    13. Biological characterization of cultured dermal papilla cells tnd hair follicle regeneration in vitro and in vivo 中华医学杂志:英文版 · 2006
    14. Study on biologic characteristics of cultured dermal papilla cells and hair follicle regeneration in vitro and in vivo Chinese Journal of Dermatology · 2006
    15. Intrinsic activation of cell growth and differentiation in ex vivo cultured human hair follicles by a transient endogenous production of ROS Scientific reports · 2019 · 9 citations
    16. 773 Generation of human hair follicle organoids in vitro and ex vivo by co-culture of primary human hair matrix keratinocytes and dermal papilla fibroblasts Journal of Investigative Dermatology · 2020
    17. Dedifferentiation of committed epithelial cells into stem cells in vivo Nature · 2013 · 551 citations
    18. 613 Assessment of the induction and morphogenic potential of human hair matrix cells and dermal papilla fibroblasts ex vivo Journal of Investigative Dermatology · 2019 · 1 citations
    19. Limitations of human occipital scalp hair follicle organ culture for studying the effects of minoxidil as a hair growth enhancer Experimental Dermatology · 2004 · 30 citations
    20. Skin and hair on-a-chip: in vitro skin models versus ex vivo tissue maintenance with dynamic perfusion Lab on a Chip · 2013 · 208 citations
    21. CellTissueScience 2018: Innovations in regenerative medicine that improve the results of stem cell treatment: 3D cultivation, in vivo modeling of stem cell niche and prenatal stem cells exosomes_AbhijitBopardikar_ReeLabs Pvt. Ltd., India Cell & developmental biology · 2019
    22. Hair follicle neogenesis induced by cultured human scalp dermal papilla cells Regenerative Medicine · 2009 · 63 citations
    23. Modeling aging in a culture dish: towards the development of more sophisticated in vitro models of human skin aging Ageing Research Reviews · 2026
    24. A umbilical cord blood-derived exosome product simulates hair growth through laminin V and collagen XVII: an ex vivo study Archives of Dermatological Research · 2025
    25. Effect of Orthosiphon stamineus plant extract on in vitro dermal papilla cell proliferation and ex vivo hair growth Deleted Journal · 2017 · 1 citations
    26. Clonal Growth of Dermal Papilla Cells in Hydrogels Reveals Intrinsic Differences between Sox2-Positive and -Negative Cells In Vitro and In Vivo Journal of Investigative Dermatology · 2011 · 68 citations
    27. In vitro models to study hair follicle generation Elsevier eBooks · 2017 · 5 citations
    28. Glycogen Phosphorylase Inhibitor Promotes Hair Growth via Protecting from Oxidative-Stress and Regulating Glycogen Breakdown in Human Hair follicles Biomolecules & Therapeutics · 2024
    29. Repigmentation of Gray Hair After Thyroid Hormone Treatment 2007 · 6 citations
    30. [Hair follicle regeneration after implantation of hair follicle cells into subcutis of nude mice]. PubMed · 2004