Minoxidil-Coated Lysozyme-Shelled Microbubbles Combined With Ultrasound for the Enhancement of Hair Follicle Growth: Efficacy In Vitro and In Vivo

The study demonstrated that the combination of minoxidil-coated lysozyme-shelled microbubbles (Mx-LyzMBs) and ultrasound significantly enhances hair follicle growth. The research was conducted both in vitro and in vivo, with the in vivo study involving 30 mice. The loading efficiency of minoxidil on the microbubbles was 20.3%. In hair follicle cell culture experiments, the lengths of hair follicle cells were significantly enhanced in the ultrasound and Mx-LyzMBs group compared to other groups. In animal experiments, the growth rates in the ultrasound and Mx-LyzMBs group increased by 19.4% and 65.7% at days 10 and 14, respectively. The study also found that the application of ultrasound energy enhanced the release of Minoxidil from the LyzMBs by 2.2–10.2%. The results suggest that this novel approach could be a promising strategy for treating hair loss.