A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal-Epidermal Composites Using Non-Adherent Microwell Arrays

This study introduces a novel method for creating dermal papilla spheroids using non-adherent microwell arrays, which are then integrated into dermal–epidermal composites (DECs) for potential use in skin grafting. Human dermal papilla cells (HDPCs) were cultured to form spheroids in microwell arrays, which were then combined with human keratinocytes to form DECs. These composites were grafted onto immunocompromised mice, showing successful graft acceptance and hair follicle formation over 10 weeks. The technique demonstrated increased expression of key HDPC markers essential for hair follicle development, offering a promising alternative to traditional skin grafting methods by potentially reducing preparation time and improving functional outcomes in wound healing.