Optimization of Hair Follicle Spheroids for Hair-On-A-Chip

    January 2024 in “ Biomaterials Science
    Subin Jeong, Hyeon‐Min Nam, Gun Yong Sung
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    TLDR The method could grow hair in lab settings without using animals.
    The study addresses limitations in current hair follicle research models by optimizing hair follicle spheroids for a "hair-on-a-chip" system. Researchers transfected hair growth factors into dermal papilla cells (DPCs) and cultured them with keratinocytes (KCs), optimizing cell numbers and culture periods. The study examined the expression of hair growth factors in spheroids with or without human umbilical vein endothelial cells (HUVECs). In spheroids co-cultured with DPCs, KCs, and HUVECs for 21 days, specific hair growth markers were observed, suggesting that this method could produce full-thickness skin equivalents with hair in vitro, potentially eliminating the need for animal transplantation.
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