Microtechnology-Based Methods for Organoid Models

The document reviewed the advancements in microtechnology-based methods for producing organoids and spheroids, highlighting their superiority over traditional 2D cell cultures and animal models in studying human diseases and development. It noted that while numerous production methodologies existed, many faced challenges related to complexity, throughput, and reproducibility. Microfabricated and microfluidic platforms were identified as promising solutions to these issues, as they improved nutrient delivery, allowed for size-controlled culture areas, and enabled more uniform and cost-effective production. The review focused on how techniques like lithography, microcontact printing, and microfluidic systems could enhance the application of organoids and spheroids in medical research.