MEF/KSF-Conditioned Culture Medium: An Effective Method for In Vitro Culture of Mouse Dermal Papilla Cells with Osteogenic Differentiation Potential

June 2021 in “ Experimental and Therapeutic Medicine ”

Liang Xu, Wenlan Gao, Shanshan Bai, Huichuan Duan, Xiaogang Pan, Wei Wu

mouse dermal papilla cells hair follicle stem cells mouse embryonic fibroblast keratinocyte serum-free medium osteogenic differentiation hair follicle dermal papilla mesenchymal stem cells

TLDR MEF/KSF-conditioned medium effectively grows mouse hair follicle stem cells with bone-forming potential.

The study investigated the in vitro culture and osteogenic differentiation potential of mouse hair follicle stem cells using different culture systems. It was found that hair follicle-derived stem cells cultured in mouse embryonic fibroblast (MEF)/keratinocyte serum-free medium (KSF)-conditioned medium exhibited the highest proliferative ability and stronger osteogenic differentiation potential compared to non-induced groups. These cells, derived from the hair follicle dermal papilla, demonstrated biological characteristics similar to dermal papilla cells, suggesting that MEF/KSF-conditioned medium was an effective method for amplifying hair follicle-derived mesenchymal stem cells with osteogenic potential.

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