A Practical Guide for the Study of Human and Murine Sebaceous Glands In Situ

    September 2013 in “ Experimental Dermatology ”
    Eleanor Hinde, Iain S. Haslam, Marlon R. Schneider, Ewan A. Langan, Jennifer E. Kloepper, Carolin Schramm, Christos C. Zouboulis, Ralf Paus
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    TLDR The guide explains how to study human and mouse sebaceous glands using various staining and imaging techniques, and emphasizes the need for standardized assessment methods.
    The document from 2013 serves as a guide for studying human and murine sebaceous glands (SGs) in situ, providing techniques for visualizing and quantifying SGs and sebocytes. It recommends using routine histochemical stains like H&E, PAS, and lipid-specific stains, as well as immunohistochemical and immunofluorescent markers to study differentiating sebocytes and progenitor cells. The guide highlights the importance of quantitative morphometry for assessing SG architecture and suggests using three-dimensional methods and image analysis software for more accurate analysis. It also discusses the use of proliferation markers like Ki67, BrdU, and EdU, and apoptosis markers such as TUNEL and cleaved caspase 3, while noting the limitations of some of these markers. The document encourages the standardization of SG assessment criteria for future clinical trials and acknowledges technical and financial support, with no conflicts of interest declared.
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