Suppression of Wnt/β-Catenin Signaling by EGF Receptor Is Required for Hair Follicle Development
November 2018
in “
Molecular Biology of the Cell
”
Wnt/β-catenin signaling Epidermal Growth Factor Receptor EGFR hair follicle development kinase activity Wnt gene transcription β-catenin signaling hair follicle defects proliferation apoptosis keratinocytes Wnt antagonist sFRP1 autocrine loops Wnt signaling hair growth cell proliferation cell death skin cells Wnt blocker secreted frizzled-related protein 1
TLDR Blocking Wnt/β-catenin signaling with EGF receptor is necessary for proper hair growth.
The document details a study that explored the role of the Epidermal Growth Factor Receptor (EGFR) in hair follicle development in mice, focusing on its interaction with Wnt/β-catenin signaling. The researchers found that EGFR kinase activity is necessary to suppress Wnt gene transcription and β-catenin signaling, which is essential for normal hair follicle development. Mice with kinase-inactive EGFR showed increased Wnt transcripts, hyperactivation of β-catenin signaling, and hair follicle defects, including increased proliferation and apoptosis. The study demonstrated that EGFR activation leads to the suppression of Wnt mRNA synthesis in primary keratinocytes and that overexpression of the Wnt antagonist sFRP1 can partially rescue the hair follicle defects in EGFR-deficient mice. The findings suggest that EGFR autocrine loops are crucial for controlling Wnt signaling, balancing proliferation and differentiation during hair follicle development. The experiments involved at least 3 mice, with over 20 hair follicles counted per mouse, and 25-150 cells counted per hair follicle, providing a moderate sample size for the study.
