Expression Profile Analysis of Dermal Papilla Cells mRNA in Response to WNT10B Treatment

The study investigated the effects of WNT10B on dermal papilla cells (DPCs) and its potential for alopecia therapy. Researchers found that WNT10B played a crucial role in the proliferation and maintenance of these cells, promoting hair follicle growth and differentiation. RNA sequencing revealed 1,073 upregulated and 451 downregulated genes in WNT10B-treated DPCs. WNT10B treatment decreased translation initiation, elongation, and termination, as well as RNA metabolic processes, while downregulating the ribosome biogenesis pathway and decreasing protein synthesis. Additionally, WNT10B activated the PI3K/Akt signaling pathway, which is essential for cellular processes like proliferation and hair growth. These findings suggested that WNT10B might maintain the stemness of dermal papilla cells by reducing protein synthesis rates, offering insights into developing therapies for hair follicle regeneration and alopecia treatment.