Expression Profile Analysis of Dermal Papilla Cells mRNA in Response to WNT10B Treatment

December 2019 in “ Experimental and Therapeutic Medicine ”

Qiang Zhou, Yinjing Song, Qiaoli Zheng, Rui Han, Hao Cheng

WNT10B dermal papilla cells hair follicle development hair growth regulation RNA-sequencing reverse-transcription quantitative PCR Gene Ontology KEGG pathway phosphoinositide 3-kinase/protein kinase B signaling pathway DPCs RT-qPCR GO terms PI3K/AKT signaling pathway

Studysummary This study examined the effects of WNT10B on dermal papilla cells in vitro, finding that it alters gene expression, decreases protein synthesis, and upregulates a specific signaling pathway, potentially influencing hair follicle morphogenesis. Our plain-language summary of this paper — not a Tressless recommendation.

The study investigated the effects of WNT10B on dermal papilla cells (DPCs) and its potential for alopecia therapy. Researchers found that WNT10B played a crucial role in the proliferation and maintenance of these cells, promoting hair follicle growth and differentiation. RNA sequencing revealed 1,073 upregulated and 451 downregulated genes in WNT10B-treated DPCs. WNT10B treatment decreased translation initiation, elongation, and termination, as well as RNA metabolic processes, while downregulating the ribosome biogenesis pathway and decreasing protein synthesis. Additionally, WNT10B activated the PI3K/Akt signaling pathway, which is essential for cellular processes like proliferation and hair growth. These findings suggested that WNT10B might maintain the stemness of dermal papilla cells by reducing protein synthesis rates, offering insights into developing therapies for hair follicle regeneration and alopecia treatment.

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