Bioorthogonal Click-Engineered Microneedle Patch Enables Adeno-Associated Virus-Mediated Dermal Acidic Fibroblast Growth Factor Expression for Hair Loss Treatment

The study introduces a bioorthogonal click-engineered microneedle patch (ClickMNP) designed for the treatment of hair loss by delivering adeno-associated virus encoding acidic fibroblast growth factor (AAV-aFGF) directly into the skin. This innovative approach addresses the limitations of aFGF's poor bioavailability and rapid degradation. The ClickMNP facilitates sustained transgene expression, enhancing hair follicle regeneration, dermal angiogenesis, and prolonging the anagen phase in murine alopecia models. The patch outperforms traditional methods, maintaining follicle viability and promoting regrowth through repeated hair removal cycles. The study highlights the biocompatibility and effectiveness of this method, suggesting it as a promising solution for hair loss and other skin-related conditions.