Isolation, Expansion, And Characterization Of Rat Hair Follicle Stem Cells And Their Secretome: Insights Into Wound Healing Potential

December 2024 in “ Biomedicines ”

Patrícia Sousa, Bruna Lopes, Ana Catarina Sousa, André Coelho, Alícia Moreira, Alexandra Rêma, Maria Gonçalves-Maia, Irina Amorim, Rui Alvites, Nuno Alves, Stefano Geuna, Ana Colette Maurício

hair follicle stem cells secretome immunohistochemical analysis real-time PCR tri-differentiation capacity biofactors wound healing regenerative medicine skin regeneration

TLDR Rat hair follicle stem cells may help heal skin wounds.

This study presents a detailed methodology for isolating, culturing, and characterizing hair follicle stem cells from Sprague–Dawley rats, highlighting their potential in skin regeneration. The research confirms the identity of these cells through immunohistochemical analysis and real-time PCR, assessing their growth kinetics, colony formation, and tri-differentiation capacity. The secretome of these cells, rich in biofactors with wound healing properties, was also analyzed. The findings suggest that rat hair follicle stem cells could be a valuable resource for regenerative medicine, particularly in therapeutic interventions for skin regeneration.

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Research cited in this study

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research Applications of Mesenchymal Stem Cells in Skin Regeneration and Rejuvenation

76 citations, February 2021 in “International Journal of Molecular Sciences”

Mesenchymal stem cells show potential for skin healing and anti-aging, but more research is needed for safe use, especially regarding stem cells from induced pluripotent sources.

research Epidermal Stem Cells in Hair Follicle Cycling and Skin Regeneration: A View From the Perspective of Inflammation

18 citations, November 2020 in “Frontiers in Cell and Developmental Biology”

Inflammation plays a key role in activating skin stem cells for hair growth and wound healing, but more research is needed to understand how it directs cell behavior.

research Tp63-Expressing Adult Epithelial Stem Cells Cross Lineage Boundaries Revealing Latent Hairy Skin Competence

8 citations, November 2020 in “Nature Communications”

Adult stem cells with Tp63 can form hair and skin cells when placed in new skin, showing they have hidden abilities for skin repair.

Locally Delivered Umbilical Cord Mesenchymal Stromal Cells Reduce Chronic Inflammation in Long-Term Nonhealing Wounds: A Randomized Study

research Locally Delivered Umbilical Cord Mesenchymal Stromal Cells Reduce Chronic Inflammation in Long-Term Nonhealing Wounds: A Randomized Study

14 citations, February 2020 in “Stem Cells International”

Umbilical cord cells safely improve healing in long-term nonhealing wounds better than a placebo.

research Skin Stem Cells, Their Niche, and Tissue Engineering Approach for Skin Regeneration

14 citations, January 2019 in “Advances in experimental medicine and biology”

Skin stem cells are promising for healing wounds and skin regeneration due to their accessibility and regenerative abilities.

research Mesenchymal Stem Cell Secretome: Toward Cell-Free Therapeutic Strategies in Regenerative Medicine

773 citations, August 2017 in “International Journal of Molecular Sciences”

The secretions of mesenchymal stem cells could be used for healing without using the cells themselves.

research Stem Cells in Skin Regeneration, Wound Healing, and Their Clinical Applications

232 citations, October 2015 in “International journal of molecular sciences”

Stem cells are crucial for skin repair and new treatments for chronic wounds.

research Stem Cell Dynamics in the Hair Follicle Niche

135 citations, December 2013 in “Seminars in Cell & Developmental Biology”

Stem cells in the hair follicle are regulated by their surrounding environment, which is important for hair growth.

research Bulge Cells of Human Hair Follicles: Segregation, Cultivation, and Properties

27 citations, January 2006 in “Colloids and Surfaces B: Biointerfaces”

Researchers found that bulge cells from human hair can grow quickly in culture and have properties of hair follicle stem cells, which could be useful for skin treatments.

research Characterization and Isolation of Stem Cell-Enriched Human Hair Follicle Bulge Cells

550 citations, December 2005 in “The Journal of clinical investigation/The journal of clinical investigation”

Researchers successfully isolated and identified key markers of stem cell-enriched human hair follicle bulge cells.

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in Microneedling 18 upvotes 1 year ago

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