Bulge Cells of Rat Hair Follicles: Isolation, Cultivation, Morphological and Biological Features

May 2010

Maliheh Nobakht, Nowruz Najafzadeh, Manouchehr Safari, Nahid Rahbar Roshandel, Hamdollah Delaviz, Mohammad Taghi Joghataie, Mehrdad Bakhtiyari, Sara Asalgoo, Farid Safar

bulge cells hair follicles DMEM/F12 medium neural progenitor marker nestin βIII-Tubulin RIP stem cell characteristics

TLDR Rat hair follicle bulge cells can become nerve and glial cells, showing potential for neuroprotection.

The study aimed to isolate and culture bulge cells from rat hair follicles to explore their morphological and biological features. Researchers successfully cultured these cells using DMEM/F12 medium with supplements and observed their proliferation by the 4th day. The bulge cells expressed the neural progenitor marker nestin before differentiation, and upon differentiation, they expressed βIII-Tubulin and RIP, indicating their potential to differentiate into neural and glial lineages. This demonstrated that bulge cells from rat hair follicles possessed stem cell characteristics and could potentially be used for neuroprotective applications.

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Bulge Cells of Rat Hair Follicles: Isolation, Cultivation, Morphological and Biological Features

Research cited in this study

research Uncomplicated differentiation of stem cells into bipolar neurons and myelinating glia

research Multipotent hair follicle stem cells promote repair of spinal cord injury and recovery of walking function

research Stem Cell Niches

research Neural Potential of a Stem Cell Population in the Hair Follicle

research Bulge Cells of Human Hair Follicles: Segregation, Cultivation, and Properties

research Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves

research Long-Term Renewal of Hair Follicles from Clonogenic Multipotent Stem Cells

research Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons

research A Review: The Location, Molecular Characterisation and Multipotency of Hair Follicle Epidermal Stem Cells

research Nestin expression in hair follicle sheath progenitor cells

research Morphogenesis and Renewal of Hair Follicles from Adult Multipotent Stem Cells

research The Biology of Hair Follicles

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