The Overexpression of R-Spondin 3 Affects Hair Morphogenesis and Development Along with the Formation and Maturation of Hair Follicle Stem Cells

September 2024 in “ Frontiers in Physiology ”

Alicja Olczak, Tomasz D. Pieczonka, Szymon Ławicki, Konrad Łukaszyk, Anna Puławska-Czub, Linda Cambier, Krzysztof Kobielak

R-spondin 3 Rspo3 hair morphogenesis hair development hair follicle stem cells HFSCs anagen catagen Wnt-dependent hair types hair matrix progenitor cells keratinocytes

TLDR Overexpression of R-spondin 3 leads to sparse hair and impaired hair regeneration.

The study investigated the effects of R-spondin 3 (Rspo3) overexpression on hair morphogenesis and development using a transgenic mice model. The overexpression of Rspo3 led to sparse hair and bald areas due to reduced proliferation and increased apoptosis of hair matrix progenitor cells, causing a premature transition from anagen to catagen and a shortened hair growth phase. Although Rspo3GOF mice showed induction of certain Wnt-dependent hair types, the overall hair amount was reduced. The study also found delayed pre-bulge formation and prolonged immaturity of hair follicle stem cells (HFSCs), impairing hair regeneration. In vitro studies on keratinocytes showed that Rspo3 overexpression inhibited proliferation, migration, and colony formation. These findings highlight Rspo3's role in regulating hair morphogenesis, development, and HFSC maturation, affecting hair regeneration.

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