Towards Developing an Organotypic Model for the Preclinical Study and Manipulation of Human Hair Matrix-Dermal Papilla Interactions

January 2021 in “ Archives of dermatological research ”

Christopher I. Platt, Jérémy Chéret, Ralf Paus

hair follicles dermal papilla fibroblasts 3-dimensional spheroids plucked anagen hair shafts hair follicle inductivity markers alkaline phosphatase versican noggin keratin-85 apoptotic cells TUNEL HFs DP DPS HS ALP K85

TLDR The study created a new model to better understand human hair growth and health.

The study developed a novel human hair research model to address the limitations of previous in vitro models that failed to accurately replicate the complex interactions in hair follicles (HFs). This new model utilized dermal papilla (DP) fibroblasts cultured as 3-dimensional spheroids (DPS) in combination with plucked anagen hair shafts (HS). The DPS were found to express hair follicle inductivity markers such as alkaline phosphatase (ALP), versican, and noggin, while the plucked HS retained significant remnants of the anagen hair matrix. When DPS and HS were cultured together, the DPS adhered to and surrounded the HS, enhancing the expression of the differentiation marker keratin-85 (K85) in the HS (p < 0.0001) and reducing the percentage of apoptotic (TUNEL +) cells in the proximal HS (p = 0.0508). This model represents a potentially physiologically relevant method for studying hair follicle differentiation in the human anagen hair matrix.

View this study on link.springer.com →

Discuss this study in the Community →

Cited in this study

14 / 14 results

research Overexpression of Alkaline Phosphatase Improves the Hair-Inductive Capacity of Cultured Human Dermal Papilla Spheres

13 citations , September 2019 in “Journal of Dermatological Science”

Increasing alkaline phosphatase in human skin cells helps to grow more hair.

research Tissue Engineering of Human Hair Follicles Using a Biomimetic Developmental Approach

184 citations , December 2018 in “Nature Communications”

Researchers created human hair follicles using a new method that could help treat hair loss.

research Restoration of Hair-Inductive Activity of Cultured Human Follicular Keratinocytes by Co-Culturing with Dermal Papilla Cells

14 citations , October 2018 in “Biochemical and Biophysical Research Communications”

Growing hair cells with dermal cells can potentially treat hair loss.

research Olfactory Receptor OR2AT4 Regulates Human Hair Growth

82 citations , September 2018 in “Nature Communications”

A certain smell receptor in hair follicles can affect hair growth when activated by a synthetic sandalwood scent.

research Establishment of an In Vitro Organoid Model of Dermal Papilla of Human Hair Follicle

44 citations , June 2018 in “Journal of Cellular Physiology”

Researchers developed a 3D model of human hair follicle cells that can help understand hair growth and test new hair loss treatments.

research Identifying Novel Strategies for Treating Human Hair Loss Disorders: Cyclosporine A Suppresses the Wnt Inhibitor, SFRP1, in the Dermal Papilla of Human Scalp Hair Follicles

68 citations , May 2018 in “PLOS Biology”

Cyclosporine A may help treat hair loss by blocking a protein that inhibits hair growth.

research Characterization of Cell Cycle Arrest and Terminal Differentiation in a Maximally Proliferative Human Epithelial Tissue: Lessons from the Human Hair Follicle Matrix

30 citations , September 2017 in “European Journal of Cell Biology”

CIP/KIP proteins help stop cell division and support hair growth.

research Methods for the Isolation and 3D Culture of Dermal Papilla Cells from Human Hair Follicles

55 citations , June 2017 in “Experimental Dermatology”

The document describes a way to isolate and grow human hair follicle cells in 3D to help study hair growth.

research Activation of Beta-Catenin Signaling in CD133-Positive Dermal Papilla Cells Drives Postnatal Hair Growth

26 citations , July 2016 in “PLOS ONE”

Activating β-catenin in certain skin cells speeds up hair growth in mice.

research Human Hair Follicle Organ Culture: Theory, Application, and Perspectives

130 citations , November 2015 in “Experimental Dermatology”

Human hair follicle organ culture is a useful model for hair research with potential for studying hair biology and testing treatments.

research Regenerative Medicine and Hair Loss: How Hair Follicle Culture Has Advanced Our Understanding of Treatment Options for Androgenetic Alopecia

23 citations , January 2014 in “Regenerative Medicine”

Hair follicle culture helps develop new treatments for hair loss.

research Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

256 citations , October 2013 in “Proceedings of the National Academy of Sciences of the United States of America”

Growing human skin cells in a 3D environment can stimulate new hair growth.

research Strategies to Enhance Epithelial-Mesenchymal Interactions for Human Hair Follicle Bioengineering

57 citations , May 2013 in “Journal of Dermatological Science”

Improving the environment and cell interactions is key for creating human hair in the lab.

research A Human Folliculoid Microsphere Assay for Exploring Epithelial-Mesenchymal Interactions in the Human Hair Follicle

71 citations , April 2009 in “The journal of investigative dermatology/Journal of investigative dermatology”

HFMs can help study hair growth and test potential hair growth drugs.

7 / 7 results

research Possible Cellular Communication with Human Follicle Dermal Papilla Cells via Secretions from Human Hair Follicle Keratinocytes

January 2022 in “Journal of St. Marianna University”

Substances from human hair cells can affect hair loss-related genes, potentially leading to new treatments for baldness.

research A Model System to Analyze the Ability of Human Keratinocytes to Form Hair Follicles

15 citations , May 2014 in “Experimental Dermatology”

Scientists developed a system to study human hair growth using skin cells, which could help understand hair development and improve skin substitutes for medical use.

research Review of Hair Follicle Dermal Cells

321 citations , January 2010 in “Journal of Dermatological Science”

Dermal cells are key in controlling hair growth and could potentially be used in hair loss treatments, but more research is needed to improve hair regeneration methods.