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    GlossaryDirect Immunofluorescence

    fluorescent antibodies bind directly to tissue samples for visualization

    Direct Immunofluorescence (DIF) is a laboratory technique used to detect the presence of specific antibodies or antigens in tissue samples. In this method, fluorescently-labeled antibodies bind directly to the target molecules, allowing them to be visualized under a fluorescence microscope. This technique is often used in diagnosing autoimmune diseases and certain skin conditions, including some forms of alopecia.

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