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    GlossaryIndirect Immunofluorescence

    technique using fluorescent-tagged antibodies to detect specific proteins

    Indirect Immunofluorescence (IIF) is a laboratory technique used to detect specific antibodies or antigens in a sample. It involves applying a primary antibody to the target antigen, followed by a secondary antibody that is tagged with a fluorescent dye. When viewed under a fluorescence microscope, the areas where the antibodies have bound will glow, allowing researchers to visualize the presence and location of the target molecules. This method is widely used in medical diagnostics and research, including the study of autoimmune diseases and infections.

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