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Glossary Indirect Immunofluorescence

technique using fluorescent-tagged antibodies to detect specific proteins

Indirect Immunofluorescence (IIF) is a laboratory technique used to detect specific antibodies or antigens in a sample. It involves applying a primary antibody to the target antigen, followed by a secondary antibody that is tagged with a fluorescent dye. When viewed under a fluorescence microscope, the areas where the antibodies have bound will glow, allowing researchers to visualize the presence and location of the target molecules. This method is widely used in medical diagnostics and research, including the study of autoimmune diseases and infections.

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research Clinical Significance of Dense Fine Speckled Pattern in Anti-nuclear Antibody Test using Indirect Immunofluorescence Method

28 citations , April 2009 in “Annals of laboratory medicine”

This study found that the DFS pattern occurs in about 28% of ANA-positive cases and is relatively frequent in autoimmune diseases, contrary to previous observations suggesting no link with these diseases.

research Skin biopsy

106 citations , December 2015 in “Journal of The American Academy of Dermatology”

This article reviews common pitfalls in biopsy site selection and technique that can lead to misdiagnosis of various skin conditions, highlighting the importance of proper biopsy practices for improving diagnostic accuracy.

research The Expression of Vitamin D-Upregulated Protein 1 in Skin and its Interaction with Sciellin in Cultured Keratinocytes

18 citations , September 2003 in “The journal of investigative dermatology/Journal of investigative dermatology”

This study suggests that vitamin D-upregulated protein 1 (VDUP1) might play a unique role in regulating the differentiation of epidermal cells.

research Cultured nail keratinocytes express hard keratins characteristic of nail and hair in vivo

16 citations , August 1992 in “Archives of dermatological research”

Lab-grown nail cells show characteristics similar to natural nail and hair.

research Diagnostic challenge: Loose anagen hair syndrome

1 citations , April 2016 in “Journal of The American Academy of Dermatology”

This study found that patients with androgenetic alopecia experienced more severe symptoms than those with alopecia areata, influencing their quality of life differently based on various patient characteristics.

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