Analysis of the MicroRNA Expression Profile of Normal Human Dermal Papilla Cells Treated with 5α-Dihydrotestosterone

March 2012 in “ Molecular Medicine Reports ”

Myung J. Lee, Hwa Jun, Kyung Mi Lim, Ok‐Kyu Lee, Seunghee Bae, Chun‐Ho Kim, Kee‐Ho Lee, Yu Na Lee, Kyu Joong Ahn, Sungkwan An

5α-dihydrotestosterone DHT normal human dermal papilla cells nHDPCs reactive oxygen species ROS senescence microRNAs miRNAs miRNA-125a-3p miR-485-5p androgenetic alopecia cell proliferation cell migration hair follicle cells oxidative stress hair loss male pattern baldness

TLDR DHT affects hair follicle cells by changing microRNA levels, leading to less cell growth and more cell death.

The study analyzed the impact of 5α-dihydrotestosterone (DHT) on normal human dermal papilla cells (nHDPCs), discovering that DHT treatment led to decreased cell growth, cell death, G2 cell cycle arrest, increased reactive oxygen species (ROS) production, and senescence. It was found that 61 microRNAs (miRNAs) were significantly affected, with 55 being upregulated and 6 downregulated. The altered miRNAs included miRNA-125a-3p and miR-485-5p, which are known to repress cell proliferation and migration. These changes in miRNA expression suggest potential mechanisms for the effects of DHT on nHDPCs, which may contribute to the development of androgenetic alopecia. The study provided evidence that miRNA regulation is involved in the cellular response to DHT treatment in hair follicle cells.

View this study on spandidos-publications.com →

Discuss this study in the Community →

Research cited in this study

12 / 12 results

Update on the Genetics of Androgenetic Alopecia, Female Pattern Hair Loss, and Alopecia Areata: Implications for Molecular Diagnostic Testing

research Update on the Genetics of Androgenetic Alopecia, Female Pattern Hair Loss, and Alopecia Areata: Implications for Molecular Diagnostic Testing

11 citations, November 2012 in “Seminars in Cutaneous Medicine and Surgery”

Genetic factors affect hair loss, and molecular testing may help predict, diagnose, and treat it.

research Molecular Basis of Androgenetic Alopecia: From Androgen to Paracrine Mediators Through Dermal Papilla

122 citations, November 2010 in “Journal of Dermatological Science”

Male pattern baldness involves hormones and cell signals affecting hair growth.

research Review of Hair Follicle Dermal Cells

321 citations, December 2009 in “Journal of Dermatological Science”

Dermal cells are key in controlling hair growth and could potentially be used in hair loss treatments, but more research is needed to improve hair regeneration methods.

research Androgens and Hair Loss: An In-Depth Analysis of Androgenetic Alopecia

52 citations, June 2009 in “Current Opinion in Endocrinology, Diabetes and Obesity”

The document concludes that hair transplantation and gene therapy may be important for future hair loss treatment.

research The Effect of 5-Reductase Inhibitors on Erectile Function

31 citations, September 2008 in “International Journal of Andrology”

5-alpha-reductase inhibitors may cause a low incidence of erectile dysfunction that decreases over time.

research Dihydrotestosterone-Inducible Dickkopf 1 from Balding Dermal Papilla Cells Causes Apoptosis in Follicular Keratinocytes

171 citations, July 2007 in “Journal of Investigative Dermatology”

A substance called DKK-1 increases in balding areas and causes hair cells to die when exposed to DHT.

research Effect of 5α-Dihydrotestosterone and Testosterone on Apoptosis in Human Dermal Papilla Cells

58 citations, January 2006 in “Skin Pharmacology and Physiology”

High levels of testosterone and 5α-DHT can lead to cell death in cells important for hair growth.

Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla

research Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla

208 citations, December 2003 in “Journal of Investigative Dermatology”

Certain cells from hair follicles can create new hair and contribute to hair growth when implanted in mice.

The Expression of Insulin-Like Growth Factor 1 in Follicular Dermal Papillae Correlates with Therapeutic Efficacy of Finasteride in Androgenetic Alopecia

research The Expression of Insulin-Like Growth Factor 1 in Follicular Dermal Papillae Correlates with Therapeutic Efficacy of Finasteride in Androgenetic Alopecia

49 citations, August 2003 in “Journal of The American Academy of Dermatology”

Higher IGF-1 levels in hair follicles link to better finasteride results for hair loss.

research Molecular Mechanisms of Androgenetic Alopecia

229 citations, August 2002 in “Experimental Gerontology”

AGA causes hair loss by shrinking hair follicles due to DHT binding, and can be treated with finasteride and minoxidil.

research 5α-Reductase Activity in the Human Hair Follicle Concentrates in the Dermal Papilla

41 citations, March 1998 in “Archives of Dermatological Research”

The enzyme that changes testosterone to a stronger form is mostly found in the part of the hair follicle called the dermal papilla.

research Growth Factors in Hair Organ Development and the Hair Growth Cycle

90 citations, October 1996 in “Dermatologic Clinics”

Growth factors are crucial for hair development and could help treat hair diseases.

Similar Research

5 / 1000+ results

research Epigallocatechin Gallate-Mediated Alteration of the MicroRNA Expression Profile in 5α-Dihydrotestosterone-Treated Human Dermal Papilla Cells

16 citations, January 2016 in “Annals of Dermatology”

Green tea component EGCG may help prevent hair loss by changing microRNA levels in certain scalp cells.

research The Hair Follicle: A Paradoxical Androgen Target Organ

66 citations, January 2000 in “Hormone Research in Paediatrics”

Androgens can both promote and prevent hair growth due to differences in gene expression in hair follicles.

Androgens Affect the Activity of Human Sebocytes in Culture Depending on the Localization of the Sebaceous Glands, and Their Effect Is Antagonized by Spironolactone

research Androgens Affect the Activity of Human Sebocytes in Culture Depending on the Localization of the Sebaceous Glands, and Their Effect Is Antagonized by Spironolactone

40 citations, January 1994 in “Skin Pharmacology and Physiology”

Male hormones affect oil-producing skin cells differently based on their body location, and the drug spironolactone can reduce these effects.

research Hair Growth-Promoting Effect of Hydrangea Serrata Extract and Its Active Component Hydrangenol: In Vitro and In Vivo Study

September 2024 in “International Journal of Molecular Sciences”

Hydrangea serrata extract may promote hair growth and improve hair health.

research Alopecia: The Search for Novel Agents Continues

11 citations, August 1997 in “Expert Opinion on Therapeutic Patents”

Many potential alopecia treatments need more testing to confirm they promote acceptable hair growth with minimal side effects.