Development of Hair Follicle Dermal Papilla Spheroids Encapsulated in Alginate Hydrogel with Extracellular Matrix Protein

November 2024

Phasin Srinualchai, Thanchanok Khorporn, Patsawee Sriboonaied, Peerapat Thongnuek

dermal papilla alginate hydrogel extracellular matrix proteins calcium chloride alkaline phosphatase

Studysummary This study found that encapsulating dermal papilla spheroids in alginate hydrogel with extracellular matrix proteins increased alkaline phosphatase activity, suggesting an enhanced manipulation of dermal papilla activity. Our plain-language summary of this paper — not a Tressless recommendation.

The study explored the impact of encapsulating dermal papilla (DP) spheroids in alginate hydrogel combined with extracellular matrix (ECM) proteins on their activity. By aggregating DP cells to mimic their natural spherical structure and introducing them into an alginate solution mixed with ECM, researchers were able to form a hydrogel through crosslinking with calcium chloride. This encapsulation process resulted in elevated alkaline phosphatase (ALP) activity, a molecular marker of DP, indicating that this method can effectively manipulate DP spheroid activity.

View this study on ieeexplore.ieee.org →

Discuss this study in the Community →

Research cited in this study

10 / 10 results

Maintaining Inducibility of Dermal Follicle Cells on Silk Fibroin/Sodium Alginate Scaffold for Enhanced Hair Follicle Regeneration

research Maintaining Inducibility of Dermal Follicle Cells on Silk Fibroin/Sodium Alginate Scaffold for Enhanced Hair Follicle Regeneration

7 citations , March 2021 in “Biology”

This study found that silk fibroin/sodium alginate scaffolds seeded with dermal papilla cells may accelerate skin repair and hair follicle regeneration in a rat model.

research Interfollicular Epidermal Stem-Like Cells for the Recreation of the Hair Follicle Epithelial Compartment

14 citations , January 2021 in “Stem cell research & therapy”

This study suggests that interfollicular epidermal keratinocytes with stem-like features may serve as an alternative to follicular keratinocytes for hair regeneration by supporting hair follicle morphogenesis in vivo.

research Alginate and Its Application to Tissue Engineering

425 citations , January 2021 in “SN Applied Sciences”

This article highlights the versatility and potential of alginate and its derivatives in tissue engineering applications, discussing their properties, modification techniques, and various uses in enhancing tissue healing, bone regeneration, and cell growth.

research Characterization of Human Dermal Papilla Cells in Alginate Spheres

8 citations , October 2018 in “Applied sciences”

This study found that alginate spheres maintain dermal papilla cells in a dormant state with increased trichogenecity, suggesting potential benefits for cell therapy in androgenic alopecia.

research The Diagnosis and Treatment of Hair and Scalp Diseases

37 citations , May 2016 in “Deutsches Arzteblatt International”

This review outlines various causes and manifestations of hair loss, highlighting that each type has distinct underlying mechanisms and requires specific treatment approaches, without reporting new clinical results.

research Assembling Composite Dermal Papilla Spheres with Adipose-Derived Stem Cells to Enhance Hair Follicle Induction

37 citations , May 2016 in “Scientific Reports”

In this study, the researchers found that using a core-shell assembly of adipose-derived stem cells and dermal papilla cells reconstructed hair follicle cellular arrangements more effectively than mixed assemblies, enhancing hair formation in vivo.

research Strategies to Enhance Epithelial-Mesenchymal Interactions for Human Hair Follicle Bioengineering

57 citations , February 2013 in “Journal of Dermatological Science”

This article reviews methods to enhance epithelial–mesenchymal interactions for hair follicle bioengineering but does not present new experimental results; it emphasizes optimizing combinations for successful regeneration.

Scalable Production of Controllable Dermal Papilla Spheroids on PVA Surfaces and the Effects of Spheroid Size on Hair Follicle Regeneration

research Scalable Production of Controllable Dermal Papilla Spheroids on PVA Surfaces and the Effects of Spheroid Size on Hair Follicle Regeneration

78 citations , October 2012 in “Biomaterials”

This study found that both human and rat dermal papilla spheroids can induce hair follicle neogenesis, but larger spheroids increase inductivity without significantly affecting hair fiber diameter.

research Minoxidil Use in Dermatology, Side Effects, and Recent Patents

152 citations , April 2012 in “Recent Patents on Inflammation & Allergy Drug Discovery”

This review describes minoxidil's hair regrowth mechanism and discusses dermatological applications, but reports no new clinical results, emphasizing the need for regular use and addressing allergic reactions.

research Co-Culture of Human Hair Follicles and Dermal Papillae in a Collagen Matrix

36 citations , November 1990 in “The Journal of Dermatology”

This study found that dermal papilla cells in culture may enhance and attract follicular epithelial cell growth, suggesting their pivotal role in hair and follicle development.